Cloned (Comment) | Organism |
---|---|
- |
Enterococcus avium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | xylulose 5-phosphate does not protect the enzyme from EDTA inactivation. Addition of Mn2+ at concentrations of up to 2 mM results in complete reactivation of APDH | Enterococcus avium | |
Hg2+ | 2 mM, complete inactivation | Enterococcus avium | |
PHMB | 2 mM, complete inactivation | Enterococcus avium | |
Zn2+ | 2 mM, complete inactivation | Enterococcus avium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
NADH | 20°C, pH 7.2, cosubstrate: D-xylulose 5-phosphate | Enterococcus avium | |
0.23 | - |
D-xylulose 5-phosphate | 20°C, pH 7.2, cofactor: NADH | Enterococcus avium | |
0.24 | - |
NADPH | 20°C, pH 7.2, cosubstrate: D-xylulose 5-phosphate | Enterococcus avium | |
0.63 | - |
D-arabitol 5-phosphate | 20°C, pH 8.5, cofactor: NAD+ | Enterococcus avium | |
0.65 | - |
D-xylulose 5-phosphate | 20°C, pH 7.2, cofactor NADPH | Enterococcus avium | |
0.71 | - |
NAD+ | 20°C, pH 8.5: cosubstrate: D-arabitol 5-phosphate | Enterococcus avium | |
0.8 | - |
NAD+ | 20°C, pH 8.5, cosubstrate: D-arabitol 1-phosphate | Enterococcus avium | |
2.7 | - |
NADP+ | 20°C, pH 8.5, cosubstrate: D-arabitol 1-phosphate | Enterococcus avium | |
2.9 | - |
D-arabitol 1-phosphate | 20°C, pH 8.5, cofactor: NAD+ | Enterococcus avium | |
3.6 | - |
D-arabitol 1-phosphate | 20°C, pH 8.5, cofactor: NADP+ | Enterococcus avium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required. Pure APDH contains 4.05 ions of Mn2+ | Enterococcus avium | |
additional information | no requirement for Zn2+ | Enterococcus avium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
4 * 41000, SDS-PAGE | Enterococcus avium |
160000 | - |
non-denaturing PAGE | Enterococcus avium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Enterococcus avium | participates in arabitol catabolism via the arabitol phosphate route | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus avium | Q8KQL2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterococcus avium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol 1-phosphate + NAD+ | - |
Enterococcus avium | D-xylulose 5-phosphate + NADH + H+ | - |
r | |
D-arabitol 1-phosphate + NADP+ | - |
Enterococcus avium | D-xylulose 5-phosphate + NADPH + H+ | - |
r | |
D-arabitol 5-phosphate + NAD+ | - |
Enterococcus avium | D-ribulose 5-phosphate + NADH + H+ | - |
? | |
D-arabitol 5-phosphate + NADP+ | - |
Enterococcus avium | D-ribulose 5-phosphate + NADPH + H+ | - |
? | |
additional information | participates in arabitol catabolism via the arabitol phosphate route | Enterococcus avium | ? | - |
? | |
additional information | no activity with: xylitol 5-phosphate, D-sorbitol, D-mannitol, xylitol, erythrose 4-phosphate and ribose 5-phosphate. APDH kinetics are consistent with a ternary-complex mechanism | Enterococcus avium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41000, SDS-PAGE | Enterococcus avium |
Synonyms | Comment | Organism |
---|---|---|
APDH | - |
Enterococcus avium |
D-arabitol 1-phosphate dehydrogenase | - |
Enterococcus avium |
D-arabitol 5-phosphate dehydrogenase | - |
Enterococcus avium |
D-arabitol-phosphate dehydrogenase | - |
Enterococcus avium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.4 | reduction of D-xylulose 5-phosphate with NADH or NADPH | Enterococcus avium |
8.3 | 8.6 | oxidation of D-arabitol 1-phosphate with NAD+ or NADP+ | Enterococcus avium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH | Enterococcus avium | |
NADH | the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH | Enterococcus avium | |
NADP+ | the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH | Enterococcus avium | |
NADPH | the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH | Enterococcus avium |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Enterococcus avium | isoelectric focusing | - |
6.4 |