Cloned (Comment) | Organism |
---|---|
stable transfection of HEK-293 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
T49M | inactive. Mutation is associated with Lebr congenital amaurosis. Mutant is not able to detoxify 4-hydroxynonenal in cells | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Mus musculus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8BYK4 | Rdh12 | - |
Mus musculus | Q9QYF1 | Rdh11 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
retina | - |
Mus musculus | - |
retina | expression in inner segments, cell bodies and synaptic termini of photoreceptors | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-2-nonenal + NADPH + H+ | - |
Mus musculus | (E)-4-hydroxy-2-nonenol + NADP+ | - |
? | |
(E)-4-hydroxy-2-nonenal + NADPH + H+ | Rdh12 is able to efficiently detoxify 4-hydroxynonenal in cells, most probably through its ability to reduce it to a nontoxic alcohol | Mus musculus | (E)-4-hydroxy-2-nonenol + NADP+ | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | Rdh11 is able to efficiently detoxify 4-hydroxynonenal in cells. Rdh11 protects against 4-hydroxynonenal modification of proteins and 4-hydroxynonenal-induced apoptosis in HEK-293 cells | Mus musculus |
physiological function | Rdh12 is able to efficiently detoxify 4-hydroxynonenal in cells, most probably through its ability to reduce it to a nontoxic alcohol. Cells expressing Rdh12 show significantly less formation of Michael adducts with lysine, histidine, or cysteine residues of proteins thereby inhibiting their physiological functions. Microsomes from retinas of Rdh12 knockout mice form significantly more Michael adducts with microsomal proteins in the presence of 4-hydroxynonenal than wild-type. RDH12 also protects against light-induced apoptosis of photoreceptors | Mus musculus |