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Literature summary for 1.1.1.30 extracted from
- Occurence, purification and properties of the staphylococcal beta-hydroxybutyrate dehydrogenase (1994), Acta Microbiol. Pol., 43, 33-45.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylcholine | - |
Staphylococcus xylosus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000445 | - |
NAD+ | - |
Staphylococcus xylosus | |
| 0.00102 | - |
NADH | - |
Staphylococcus xylosus | |
| 0.00547 | - |
acetoacetate | - |
Staphylococcus xylosus | |
| 0.0391 | - |
(R)-3-hydroxybutanoate | - |
Staphylococcus xylosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Staphylococcus xylosus | 5737 | - |
| mitochondrion | - |
Staphylococcus xylosus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, more than 10fold increase in activity | Staphylococcus xylosus | |
| Cd2+ | 10 mM, 2fold increase in activity | Staphylococcus xylosus | |
| Cu2+ | 10 mM, 2fold increase in activity | Staphylococcus xylosus | |
| Mg2+ | 10 mM, more than 10fold increase | Staphylococcus xylosus | |
| Mn2+ | 10 mM, more than 10fold increase in activity | Staphylococcus xylosus | |
| Zn2+ | 10 mM, 2fold increase in activity | Staphylococcus xylosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
alpha4, 4 * 39000, SDS-PAGE | Staphylococcus xylosus |
| 68000 | - |
nondenaturing PAGE | Staphylococcus xylosus |
| 140000 | - |
gel filtration | Staphylococcus xylosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutanoate + NAD+ | Staphylococcus xylosus | - |
acetoacetate + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus xylosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate precipitation, Sephacryl S-400, S200, DEAE-Sepharose | Staphylococcus xylosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.74 | - |
- |
Staphylococcus xylosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, crude extract, partially purified enzyme, several weeks, 0% inactivation | Staphylococcus xylosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutanoate + NAD+ | - |
Staphylococcus xylosus | acetoacetate + NADH | - |
r | |
| (R)-3-hydroxybutanoate + NAD+ | - |
Staphylococcus xylosus | acetoacetate + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha4, 4 * 39000, SDS-PAGE | Staphylococcus xylosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 37 | - |
Staphylococcus xylosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 49 | 80% activity at 25°C and at 49°C | Staphylococcus xylosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
45 | stable at 0°C and 25°C for 90 min, 18% activity after 90 min at 45°C, activity restored after 60 min at 37°C, loss of activity at 60°C | Staphylococcus xylosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
acetoacetate reduction | Staphylococcus xylosus |
| 8.6 | - |
oxidation of (R)-3-hydroxybutanoate | Staphylococcus xylosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | 8.6 | acetoacetate reduction, 50% activity at pH 5.0, 66% at pH 8.6 | Staphylococcus xylosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Staphylococcus xylosus | |
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