Cloned (Comment) | Organism |
---|---|
gene mcr, recombinant expression of the Chloroflexus aurantiacus enzyme in Methylosinus trichosporium strain OB3b, subcloning in Escherichia coli strain DH5alpha | Chloroflexus aurantiacus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering of type II methanotroph Methylosinus trichosporium strain OB3b for 3-hydroxypropionic acid (3HP) production by reconstructing malonyl-CoA pathway through heterologous expression of Chloroflexus aurantiacus malonyl-CoA reductase (MCR), a bifunctional enzyme. Engineering of the supply of malonyl-CoA precursors by overexpressing endogenous acetyl-CoA carboxylase (ACC), substantially enhancing the production of 3HP. Overexpression of biotin protein ligase (BPL) and malic enzyme (NADP+-ME) leads to 22.7% and 34.5% increase, respectively, in 3HP titer in ACC-overexpressing cells. Also, the acetyl-CoA carboxylation bypass route is reconstructed to improve 3HP productivity. Coexpression of methylmalonyl-CoA carboxyltransferase (MMC) of Propionibacterium freudenreichii and phosphoenolpyruvate carboxylase (PEPC), which provides the MMC precursor, further improves the 3HP titer. The highest 3HP production of 49 mg/l in the OB3b-MCRMP strain overexpressing MCR, MMC and PEPC results in a 2.4fold improvement of titer compared with that in the only MCR-overexpressing strain. 60.59 mg/l of 3HP are obatined in 42 h using the OB3b-MCRMP strain through bioreactor operation, with a 6.36fold increase of volumetric productivity compared than that in the flask cultures | Chloroflexus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonate semialdehyde + NADPH + H+ | Chloroflexus aurantiacus | - |
3-hydroxypropanoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | Q6QQP7 | bifunctional enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonate semialdehyde + NADPH + H+ | - |
Chloroflexus aurantiacus | 3-hydroxypropanoate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MCR | - |
Chloroflexus aurantiacus |
General Information | Comment | Organism |
---|---|---|
metabolism | 3-hydroxypropionic acid (3HP) production via MCR dependent pathway, overview. The bifunctional enzyme shows malonate semialdehyde reduction activity and also malonyl-CoA reduction activity, EC 1.2.1.75 | Chloroflexus aurantiacus |