Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion at 24°C K using 0.7 M ammonium dihydrogen phosphate, 0.4 M ammonium tartrate, 0.1 M sodium citrate pH 5.6 and 10 mM cupric chloride | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
estimated by dynamic light-scattering analysis | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
metal-chelate chromatography on Ni-NTA resin and size exclusion chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
erythronate-4-phosphate + NAD+ | - |
Pseudomonas aeruginosa | 3-hydroxy-4-phospho-hydroxy-alpha-ketobutyrate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42 067, enzyme including a C-terminal tag, enzyme consists of two identical 380-residue subunits | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
erythronate-4-phosphate dehydrogenase | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |