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Literature summary for extracted from

  • Kubota, K.; Nagata, K.; Miyazono, K.; Toyama, H.; Matsushita, K.; Tanokura, M.
    Purification, crystallization and preliminary X-ray analysis of L-sorbose reductase from Gluconobacter frateurii complexed with L-sorbose or NADPH (2009), Acta Crystallogr. Sect. F, 65, 562-564.
    View publication on PubMedView publication on EuropePMC


Cloned (Comment) Organism
into the HindIII/NotI site of pET-28a(+) plasmid, expression construct is designed to overexpress only L-sorbose reductase protein (residues 1-263), overexpressed in Escherichia coli BL21 (DE3) cells Gluconobacter frateurii

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with L-sorbose or NADPH using the sitting-drop vapour-diffusion method, at 20┬░C, to 2.38 and 1.90 A resolution, respectively. Crystal of the L-sorbose reductase-L-sorbose complex belongs to space group C2221, with unit-cell parameters a=124.2, b=124.1, c=60.8 A. The crystal of the L-sorbose reductase-NADPH complex belongs to space group P21, with unit-cell parameters a=124.3, b=61.0, c=124.5A, beta= 89.99┬░. The crystals contain two and eight molecules, respectively, in the asymmetric unit Gluconobacter frateurii


Organism UniProt Comment Textmining
Gluconobacter frateurii A4PB64

Purification (Commentary)

Purification (Comment) Organism
by sonication, centrifugation and gel filtration Gluconobacter frateurii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-sorbose + NADPH + H+
Gluconobacter frateurii L-sorbitol + NADP+


Synonyms Comment Organism
L-sorbose reductase
Gluconobacter frateurii


Cofactor Comment Organism Structure
Gluconobacter frateurii