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Literature summary for 1.1.1.289 extracted from

  • Kubota, K.; Nagata, K.; Miyazono, K.; Toyama, H.; Matsushita, K.; Tanokura, M.
    Purification, crystallization and preliminary X-ray analysis of L-sorbose reductase from Gluconobacter frateurii complexed with L-sorbose or NADPH (2009), Acta Crystallogr. Sect. F, 65, 562-564.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
into the HindIII/NotI site of pET-28a(+) plasmid, expression construct is designed to overexpress only L-sorbose reductase protein (residues 1-263), overexpressed in Escherichia coli BL21 (DE3) cells Gluconobacter frateurii

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with L-sorbose or NADPH using the sitting-drop vapour-diffusion method, at 20┬░C, to 2.38 and 1.90 A resolution, respectively. Crystal of the L-sorbose reductase-L-sorbose complex belongs to space group C2221, with unit-cell parameters a=124.2, b=124.1, c=60.8 A. The crystal of the L-sorbose reductase-NADPH complex belongs to space group P21, with unit-cell parameters a=124.3, b=61.0, c=124.5A, beta= 89.99┬░. The crystals contain two and eight molecules, respectively, in the asymmetric unit Gluconobacter frateurii

Organism

Organism UniProt Comment Textmining
Gluconobacter frateurii A4PB64
-
-

Purification (Commentary)

Purification (Comment) Organism
by sonication, centrifugation and gel filtration Gluconobacter frateurii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-sorbose + NADPH + H+
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Gluconobacter frateurii L-sorbitol + NADP+
-
?

Synonyms

Synonyms Comment Organism
L-sorbose reductase
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Gluconobacter frateurii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Gluconobacter frateurii