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Literature summary for 1.1.1.286 extracted from
- Characterization of two ?-decarboxylating dehydrogenases from Sulfolobus acidocaldarius (2016), Extremophiles, 20, 843-853.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene saci_2375, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3)Codon-Plus-RIL | Sulfolobus acidocaldarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
homoisocitrate | pH 8.0, 60°C, recombinant enzyme | Sulfolobus acidocaldarius |  |
| 0.027 | - |
isocitrate | pH 8.0, 60°C, recombinant enzyme | Sulfolobus acidocaldarius | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| homoisocitrate + NADP+ | Sulfolobus acidocaldarius | - |
2-oxoadipate + CO2 + NADPH + H+ | - |
r | |
| homoisocitrate + NADP+ | Sulfolobus acidocaldarius MW001 | - |
2-oxoadipate + CO2 + NADPH + H+ | - |
r | |
| isocitrate + NADP+ | Sulfolobus acidocaldarius | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
| isocitrate + NADP+ | Sulfolobus acidocaldarius MW001 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
| additional information | Sulfolobus acidocaldarius | Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase | ? | - |
? | |
| additional information | Sulfolobus acidocaldarius MW001 | Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | Q4J6C9 | - |
- |
| Sulfolobus acidocaldarius MW001 | Q4J6C9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3)Codon-Plus-RIL b anion exchange chromatography, ammonium sulfate fractionation, hydropobic interaction chromatography, ultrafiltration, and gel filtration | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| homoisocitrate + NADP+ | - |
Sulfolobus acidocaldarius | 2-oxoadipate + CO2 + NADPH + H+ | - |
r | |
| homoisocitrate + NADP+ | - |
Sulfolobus acidocaldarius MW001 | 2-oxoadipate + CO2 + NADPH + H+ | - |
r | |
| isocitrate + NADP+ | - |
Sulfolobus acidocaldarius | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
| isocitrate + NADP+ | - |
Sulfolobus acidocaldarius MW001 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
| additional information | Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase | Sulfolobus acidocaldarius | ? | - |
? | |
| additional information | Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase | Sulfolobus acidocaldarius MW001 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | dimer of dimers | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| isocitrate-homoisocitrate dehydrogenase | - |
Sulfolobus acidocaldarius |
| Saci_2375 | - |
Sulfolobus acidocaldarius |
| Saci_2375 | locus name | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Sulfolobus acidocaldarius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 37 | - |
homoisocitrate | pH 8.0, 60°C, recombinant enzyme | Sulfolobus acidocaldarius | |
| 61 | - |
isocitrate | pH 8.0, 60°C, recombinant enzyme | Sulfolobus acidocaldarius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Sulfolobus acidocaldarius |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | Saci_2375 is an NADP+-dependent enzyme with the ability to function as ICDH, EC 1.1.1.42, and HICDH, EC 1.1.1.87 | Sulfolobus acidocaldarius | |
| NADPH | Saci_2375 is an NADP+-dependent enzyme with the ability to function as ICDH, EC 1.1.1.42, and HICDH, EC 1.1.1.87 | Sulfolobus acidocaldarius | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the failure to isolate a knockout mutant of saci_2375 suggests the indispensable role of Saci_2375 in cell viability | Sulfolobus acidocaldarius |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2259 | - |
isocitrate | pH 8.0, 60°C, recombinant enzyme | Sulfolobus acidocaldarius | |
| 3364 | - |
homoisocitrate | pH 8.0, 60°C, recombinant enzyme | Sulfolobus acidocaldarius | |
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