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Literature summary for 1.1.1.284 extracted from

  • Ticha, T.; Sedlarova, M.; Cincalova, L.; Trojanova, Z.D.; Mieslerova, B.; Lebeda, A.; Luhova, L.; Petrivalsky, M.
    Involvement of S-nitrosothiols modulation by S-nitrosoglutathione reductase in defence responses of lettuce and wild Lactuca spp. to biotrophic mildews (2018), Planta, 247, 1203-1215 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ADH3, quantitative PCR enzyme expression analysis Lactuca sativa
quantitative PCR enzyme expression analysis Lactuca serriola
quantitative PCR enzyme expression analysis Lactuca saligna
quantitative PCR enzyme expression analysis Lactuca virosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-nitrosoglutathione + NADH + H+ Lactuca serriola
-
GSSG + ammonia + NAD+
-
ir
S-nitrosoglutathione + NADH + H+ Lactuca sativa
-
GSSG + ammonia + NAD+
-
ir
S-nitrosoglutathione + NADH + H+ Lactuca saligna
-
GSSG + ammonia + NAD+
-
ir
S-nitrosoglutathione + NADH + H+ Lactuca virosa
-
GSSG + ammonia + NAD+
-
ir

Organism

Organism UniProt Comment Textmining
Lactuca saligna A0A6S7LX43 cv. CGN 05271
-
Lactuca sativa J7GHV7 cvs. UCDM2 and Mariska
-
Lactuca serriola
-
cv. LSE/18
-
Lactuca virosa
-
cv. NVRS 10.001 602
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from leaf extract by gel filtration Lactuca serriola
native enzyme partially from leaf extract by gel filtration Lactuca sativa
native enzyme partially from leaf extract by gel filtration Lactuca saligna
native enzyme partially from leaf extract by gel filtration Lactuca virosa

Source Tissue

Source Tissue Comment Organism Textmining
leaf GSNOR and GSNO immunodetection by confocal laser scanning microscopy Lactuca serriola
-
leaf GSNOR and GSNO immunodetection by confocal laser scanning microscopy Lactuca sativa
-
leaf GSNOR and GSNO immunodetection by confocal laser scanning microscopy Lactuca saligna
-
leaf GSNOR and GSNO immunodetection by confocal laser scanning microscopy Lactuca virosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-nitrosoglutathione + NADH + H+
-
Lactuca serriola GSSG + ammonia + NAD+
-
ir
S-nitrosoglutathione + NADH + H+
-
Lactuca sativa GSSG + ammonia + NAD+
-
ir
S-nitrosoglutathione + NADH + H+
-
Lactuca saligna GSSG + ammonia + NAD+
-
ir
S-nitrosoglutathione + NADH + H+
-
Lactuca virosa GSSG + ammonia + NAD+
-
ir

Synonyms

Synonyms Comment Organism
ADH3
-
Lactuca sativa
GSNOR
-
Lactuca serriola
GSNOR
-
Lactuca sativa
GSNOR
-
Lactuca saligna
GSNOR
-
Lactuca virosa
S-nitrosoglutathione reductase
-
Lactuca serriola
S-nitrosoglutathione reductase
-
Lactuca sativa
S-nitrosoglutathione reductase
-
Lactuca saligna
S-nitrosoglutathione reductase
-
Lactuca virosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Lactuca serriola
25
-
assay at Lactuca sativa
25
-
assay at Lactuca saligna
25
-
assay at Lactuca virosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Lactuca serriola
8
-
assay at Lactuca sativa
8
-
assay at Lactuca saligna
8
-
assay at Lactuca virosa

Cofactor

Cofactor Comment Organism Structure
NADH
-
Lactuca serriola
NADH
-
Lactuca sativa
NADH
-
Lactuca saligna
NADH
-
Lactuca virosa

Expression

Organism Comment Expression
Lactuca serriola GSNOR expression, level and activity are studied in leaves of selected genotypes of lettuce (Lactuca sativa) and wild Lactuca spp. during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi). GSNOR expression is increased in all genotypes both in the early phase at 6 hpi and later phase at 72 hpi, with a high increase observed in Lactuca sativa UCDM2 responses to all three pathogens. GSNOR protein also shows two-phase increase, with highest changes in Lactuca virosa-Bremia lactucae and Lactuca sativa cv. UCDM2-Golovinomyces cichoracearum pathosystems, whereas Pseudoidium neolycopersici induces GSNOR protein at 72 hpi in all genotypes. Similarly, a general pattern of modulated GSNOR activities in response to biotrophic mildews involves a two-phase increase at 6 and 72 hpi. Lettuce downy mildew infection causes GSNOR activity slightly increased only in resistant Lactuca saligna and Lactuca virosa genotypes. But all genotypes show increased GSNOR activity both at 6 and 72 hpi by lettuce powdery mildew. GSNOR-mediated decrease of S-nitrosothiols is observed as a general feature of Lactuca spp. response to mildew infection, which is also confirmed by immunohistochemical detection of GSNOR and GSNO in infected plant tissues. GSNOR is differentially modulated in interactions of susceptible and resistant Lactuca spp. genotypes with fungal mildews and uncover the role of S-nitrosylation in molecular mechanisms of plant responses to biotrophic pathogens. S-Nitrosothiol profiles during pathogenesis and expression pattern, overview up
Lactuca sativa GSNOR expression, level and activity are studied in leaves of selected genotypes of lettuce (Lactuca sativa) and wild Lactuca spp. during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi). GSNOR expression is increased in all genotypes both in the early phase at 6 hpi and later phase at 72 hpi, with a high increase observed in Lactuca sativa UCDM2 responses to all three pathogens. GSNOR protein also shows two-phase increase, with highest changes in Lactuca virosa-Bremia lactucae and Lactuca sativa cv. UCDM2-Golovinomyces cichoracearum pathosystems, whereas Pseudoidium neolycopersici induces GSNOR protein at 72 hpi in all genotypes. Similarly, a general pattern of modulated GSNOR activities in response to biotrophic mildews involves a two-phase increase at 6 and 72 hpi. Lettuce downy mildew infection causes GSNOR activity slightly increased only in resistant Lactuca saligna and Lactuca virosa genotypes. But all genotypes show increased GSNOR activity both at 6 and 72 hpi by lettuce powdery mildew. GSNOR-mediated decrease of S-nitrosothiols is observed as a general feature of Lactuca spp. response to mildew infection, which is also confirmed by immunohistochemical detection of GSNOR and GSNO in infected plant tissues. GSNOR is differentially modulated in interactions of susceptible and resistant Lactuca spp. genotypes with fungal mildews and uncover the role of S-nitrosylation in molecular mechanisms of plant responses to biotrophic pathogens. S-Nitrosothiol profiles during pathogenesis and expression pattern, overview up
Lactuca saligna GSNOR expression, level and activity are studied in leaves of selected genotypes of lettuce (Lactuca sativa) and wild Lactuca spp. during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi). GSNOR expression is increased in all genotypes both in the early phase at 6 hpi and later phase at 72 hpi, with a high increase observed in Lactuca sativa UCDM2 responses to all three pathogens. GSNOR protein also shows two-phase increase, with highest changes in Lactuca virosa-Bremia lactucae and Lactuca sativa cv. UCDM2-Golovinomyces cichoracearum pathosystems, whereas Pseudoidium neolycopersici induces GSNOR protein at 72 hpi in all genotypes. Similarly, a general pattern of modulated GSNOR activities in response to biotrophic mildews involves a two-phase increase at 6 and 72 hpi. Lettuce downy mildew infection causes GSNOR activity slightly increased only in resistant Lactuca saligna and Lactuca virosa genotypes. But all genotypes show increased GSNOR activity both at 6 and 72 hpi by lettuce powdery mildew. GSNOR-mediated decrease of S-nitrosothiols is observed as a general feature of Lactuca spp. response to mildew infection, which is also confirmed by immunohistochemical detection of GSNOR and GSNO in infected plant tissues. GSNOR is differentially modulated in interactions of susceptible and resistant Lactuca spp. genotypes with fungal mildews and uncover the role of S-nitrosylation in molecular mechanisms of plant responses to biotrophic pathogens. S-Nitrosothiol profiles during pathogenesis and expression pattern, overview up
Lactuca virosa GSNOR expression, level and activity are studied in leaves of selected genotypes of lettuce (Lactuca sativa) and wild Lactuca spp. during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi). GSNOR expression is increased in all genotypes both in the early phase at 6 hpi and later phase at 72 hpi, with a high increase observed in Lactuca sativa UCDM2 responses to all three pathogens. GSNOR protein also shows two-phase increase, with highest changes in Lactuca virosa-Bremia lactucae and Lactuca sativa cv. UCDM2-Golovinomyces cichoracearum pathosystems, whereas Pseudoidium neolycopersici induces GSNOR protein at 72 hpi in all genotypes. Similarly, a general pattern of modulated GSNOR activities in response to biotrophic mildews involves a two-phase increase at 6 and 72 hpi. Lettuce downy mildew infection causes GSNOR activity slightly increased only in resistant Lactuca saligna and Lactuca virosa genotypes. But all genotypes show increased GSNOR activity both at 6 and 72 hpi by lettuce powdery mildew. GSNOR-mediated decrease of S-nitrosothiols is observed as a general feature of Lactuca spp. response to mildew infection, which is also confirmed by immunohistochemical detection of GSNOR and GSNO in infected plant tissues. GSNOR is differentially modulated in interactions of susceptible and resistant Lactuca spp. genotypes with fungal mildews and uncover the role of S-nitrosylation in molecular mechanisms of plant responses to biotrophic pathogens. S-Nitrosothiol profiles during pathogenesis and expression pattern, overview up

General Information

General Information Comment Organism
evolution GSNOR is a member of class III alcohol dehydrogenase family Lactuca serriola
evolution GSNOR is a member of class III alcohol dehydrogenase family Lactuca sativa
evolution GSNOR is a member of class III alcohol dehydrogenase family Lactuca saligna
evolution GSNOR is a member of class III alcohol dehydrogenase family Lactuca virosa
malfunction in leaves of Lactuca saligna during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi), the GSNOR expression is increased both in the early phase at 6 hpi and later phase at 72 hpi, with increased GSNOR-mediated decrease of S-nitrosothiols Lactuca saligna
malfunction in leaves of Lactuca sativa during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi), the GSNOR expression is highly increased both in the early phase at 6 hpi and later phase at 72 hpi, with increased GSNOR-mediated decrease of S-nitrosothiols Lactuca sativa
malfunction in leaves of Lactuca serriola during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi), the GSNOR expression is increased both in the early phase at 6 hpi and later phase at 72 hpi, with increased GSNOR-mediated decrease of S-nitrosothiols Lactuca serriola
malfunction in leaves of Lactuca virosa during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi), the GSNOR expression is increased both in the early phase at 6 hpi and later phase at 72 hpi, with increased GSNOR-mediated decrease of S-nitrosothiols Lactuca virosa
physiological function S-nitrosylation belongs to principal signalling pathways of nitric oxide in plant development and stress responses. Protein S-nitrosylation is regulated by S-nitrosoglutathione reductase (GSNOR) as a key catabolic enzyme of S-nitrosoglutathione (GSNO), the major intracellular S-nitrosothiol. GSNOR metabolizes S-nitrosoglutathione irreversibly to glutathione disulphide (GSSG) and ammonia (NH3) Lactuca serriola
physiological function S-nitrosylation belongs to principal signalling pathways of nitric oxide in plant development and stress responses. Protein S-nitrosylation is regulated by S-nitrosoglutathione reductase (GSNOR) as a key catabolic enzyme of S-nitrosoglutathione (GSNO), the major intracellular S-nitrosothiol. GSNOR metabolizes S-nitrosoglutathione irreversibly to glutathione disulphide (GSSG) and ammonia (NH3) Lactuca sativa
physiological function S-nitrosylation belongs to principal signalling pathways of nitric oxide in plant development and stress responses. Protein S-nitrosylation is regulated by S-nitrosoglutathione reductase (GSNOR) as a key catabolic enzyme of S-nitrosoglutathione (GSNO), the major intracellular S-nitrosothiol. GSNOR metabolizes S-nitrosoglutathione irreversibly to glutathione disulphide (GSSG) and ammonia (NH3) Lactuca saligna
physiological function S-nitrosylation belongs to principal signalling pathways of nitric oxide in plant development and stress responses. Protein S-nitrosylation is regulated by S-nitrosoglutathione reductase (GSNOR) as a key catabolic enzyme of S-nitrosoglutathione (GSNO), the major intracellular S-nitrosothiol. GSNOR metabolizes S-nitrosoglutathione irreversibly to glutathione disulphide (GSSG) and ammonia (NH3) Lactuca virosa