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Literature summary for 1.1.1.284 extracted from

  • Ben Ali, M.; Gonchar, M.; Gayda, G.; Paryzhak, S.; Maaref, M.A.; Jaffrezic-Renault, N.; Korpan, Y.
    Formaldehyde-sensitive sensor based on recombinant formaldehyde dehydrogenase using capacitance versus voltage measurements (2007), Biosens. Bioelectron., 22, 2790-2795.
    View publication on PubMed
Search for more literature for 1.1.1.284

Application

Application Comment Organism
analysis the enzyme is useful in a formaldehyde-selective biosensor using NAD+- and glutathione-dependent recombinant formaldehyde dehydrogenase as a bio-recognition element immobilized on the surface of Si/SiO2/Si3N4 structure Ogataea angusta

Cloned(Commentary)

Cloned (Comment) Organism
construction of a FDHoverproducing strain by insertion of the FLD1 gene with the own promoter into the integrative plasmid pYT1 containing the LEU2 gene of Saccharomyces cerevisiae as a selective marker , multi-copy integration and expression in strain NCYC 495 (leu1-1) Ogataea angusta

Protein Variants

Protein Variants Comment Organism
additional information construction of a formaldehyde-selective biosensor using NAD+- and glutathione-dependent recombinant formaldehyde dehydrogenase as a bio-recognition element immobilized on the surface of Si/SiO2/Si3N4 structure, evaluation by capacitance measurements, best sensitivity is observed in 2.5 mM borate buffer, pH 8.4 Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + glutathione + NAD+ Ogataea angusta the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione S-formylglutathione + NADH + H+
-
 ?
formaldehyde + glutathione + NAD+ Ogataea angusta Tf 11-6 the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione S-formylglutathione + NADH + H+
-
 ?
formaldehyde + glutathione + NAD+ Ogataea angusta Tf-11-6 the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione S-formylglutathione + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
 gene FLD1
-
Ogataea angusta Tf-11-6
-
 gene FLD1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from strain NCYC 495 by anion exchange chromatography Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
 purified recombinant enzyme Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + glutathione + NAD+ the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione Ogataea angusta S-formylglutathione + NADH + H+
-
 ?
formaldehyde + glutathione + NAD+ the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione Ogataea angusta Tf 11-6 S-formylglutathione + NADH + H+
-
 ?
formaldehyde + glutathione + NAD+ the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione Ogataea angusta Tf-11-6 S-formylglutathione + NADH + H+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 enzyme immobilized on the surface of Si/SiO2/Si3N4 structure Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Ogataea angusta