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Literature summary for 1.1.1.284 extracted from

  • Kato, N.
    Formaldehyde dehydrogenase from methylotrophic yeasts (1990), Methods Enzymol., 188, 455-462.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
[Candida] boidinii
Ag+
-
[Candida] boidinii
AMP
-
[Candida] boidinii
ATP
-
[Candida] boidinii
Cd2+
-
[Candida] boidinii
Cd2+
-
Komagataella pastoris
Cu2+
-
[Candida] boidinii
Cu2+
-
Komagataella pastoris
Hg2+
-
[Candida] boidinii
Hg2+
-
Komagataella pastoris
NADH competitive inhibition, product inhibition [Candida] boidinii
NADH
-
Komagataella pastoris
PCMB
-
[Candida] boidinii
PCMB
-
Komagataella pastoris
S-formylglutathione
-
[Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NAD+
-
[Candida] boidinii
0.025
-
NADH
-
[Candida] boidinii
0.12
-
S-formylglutathione
-
[Candida] boidinii
0.29
-
formaldehyde
-
[Candida] boidinii
2.8
-
methylglyoxal
-
[Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE [Candida] boidinii
82000
-
gel filtration [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + glutathione + NAD+ Ogataea angusta
-
S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ [Candida] boidinii key step of the methanol catabolism in yeast S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ [Candida] boidinii 2201 key step of the methanol catabolism in yeast S-formylglutathione + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
-
-
Ogataea angusta
-
-
-
Pichia sp.
-
NRRL-Y-11328
-
Pichia sp. NRRL-Y-11328
-
NRRL-Y-11328
-
[Candida] boidinii
-
-
-
[Candida] boidinii 2201
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Candida] boidinii

Reaction

Reaction Comment Organism Reaction ID
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H + H+ bi bi mechanism [Candida] boidinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38.3
-
-
[Candida] boidinii

Storage Stability

Storage Stability Organism
-20°C, in presence of glycerol, stable for at least 6 months [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + glutathione + NAD+
-
Komagataella pastoris S-formylglutathione + NADH + H+
-
r
formaldehyde + glutathione + NAD+
-
Ogataea angusta S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+
-
Pichia sp. S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione [Candida] boidinii S-formylglutathione + NADH + H+
-
r
formaldehyde + glutathione + NAD+ key step of the methanol catabolism in yeast [Candida] boidinii S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione [Candida] boidinii 2201 S-formylglutathione + NADH + H+
-
r
formaldehyde + glutathione + NAD+ key step of the methanol catabolism in yeast [Candida] boidinii 2201 S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+
-
Pichia sp. NRRL-Y-11328 S-formylglutathione + NADH + H+
-
?
methylglyoxal + glutathione + NAD+
-
Komagataella pastoris S-pyruvylglutathione + NADH
-
?
methylglyoxal + glutathione + NAD+ 89% of the activity with formaldehyde [Candida] boidinii S-pyruvylglutathione + NADH
-
?
methylglyoxal + glutathione + NAD+ 89% of the activity with formaldehyde [Candida] boidinii 2201 S-pyruvylglutathione + NADH
-
?
S-formylglutathione + NADH
-
Komagataella pastoris formaldehyde + glutathione + NAD+
-
r
S-formylglutathione + NADH
-
[Candida] boidinii formaldehyde + glutathione + NAD+
-
r
S-formylglutathione + NADH
-
[Candida] boidinii 2201 formaldehyde + glutathione + NAD+
-
r

Subunits

Subunits Comment Organism
dimer
-
Komagataella pastoris
dimer 2 * 43000, SDS-PAGE [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Komagataella pastoris
8
-
-
[Candida] boidinii
8
-
-
Ogataea angusta
8.5
-
-
Komagataella pastoris
8.5
-
-
[Candida] boidinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 20°C, 1 h, stable [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Komagataella pastoris
NAD+
-
[Candida] boidinii
NAD+
-
Ogataea angusta
NAD+
-
Pichia sp.
NADH
-
Komagataella pastoris
NADH
-
[Candida] boidinii
NADP+ no activity with NADP+ Komagataella pastoris
NADP+ no activity with NADP+ [Candida] boidinii