Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
[Candida] boidinii | |
Ag+ | - |
[Candida] boidinii | |
AMP | - |
[Candida] boidinii | |
ATP | - |
[Candida] boidinii | |
Cd2+ | - |
[Candida] boidinii | |
Cd2+ | - |
Komagataella pastoris | |
Cu2+ | - |
[Candida] boidinii | |
Cu2+ | - |
Komagataella pastoris | |
Hg2+ | - |
[Candida] boidinii | |
Hg2+ | - |
Komagataella pastoris | |
NADH | competitive inhibition, product inhibition | [Candida] boidinii | |
NADH | - |
Komagataella pastoris | |
PCMB | - |
[Candida] boidinii | |
PCMB | - |
Komagataella pastoris | |
S-formylglutathione | - |
[Candida] boidinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NAD+ | - |
[Candida] boidinii | |
0.025 | - |
NADH | - |
[Candida] boidinii | |
0.12 | - |
S-formylglutathione | - |
[Candida] boidinii | |
0.29 | - |
formaldehyde | - |
[Candida] boidinii | |
2.8 | - |
methylglyoxal | - |
[Candida] boidinii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | [Candida] boidinii |
82000 | - |
gel filtration | [Candida] boidinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | Ogataea angusta | - |
S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | [Candida] boidinii | key step of the methanol catabolism in yeast | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | [Candida] boidinii 2201 | key step of the methanol catabolism in yeast | S-formylglutathione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
- |
- |
Ogataea angusta | - |
- |
- |
Pichia sp. | - |
NRRL-Y-11328 | - |
Pichia sp. NRRL-Y-11328 | - |
NRRL-Y-11328 | - |
[Candida] boidinii | - |
- |
- |
[Candida] boidinii 2201 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
[Candida] boidinii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H + H+ | bi bi mechanism | [Candida] boidinii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38.3 | - |
- |
[Candida] boidinii |
Storage Stability | Organism |
---|---|
-20°C, in presence of glycerol, stable for at least 6 months | [Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | - |
Komagataella pastoris | S-formylglutathione + NADH + H+ | - |
r | |
formaldehyde + glutathione + NAD+ | - |
Ogataea angusta | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
Pichia sp. | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione | [Candida] boidinii | S-formylglutathione + NADH + H+ | - |
r | |
formaldehyde + glutathione + NAD+ | key step of the methanol catabolism in yeast | [Candida] boidinii | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione | [Candida] boidinii 2201 | S-formylglutathione + NADH + H+ | - |
r | |
formaldehyde + glutathione + NAD+ | key step of the methanol catabolism in yeast | [Candida] boidinii 2201 | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
Pichia sp. NRRL-Y-11328 | S-formylglutathione + NADH + H+ | - |
? | |
methylglyoxal + glutathione + NAD+ | - |
Komagataella pastoris | S-pyruvylglutathione + NADH | - |
? | |
methylglyoxal + glutathione + NAD+ | 89% of the activity with formaldehyde | [Candida] boidinii | S-pyruvylglutathione + NADH | - |
? | |
methylglyoxal + glutathione + NAD+ | 89% of the activity with formaldehyde | [Candida] boidinii 2201 | S-pyruvylglutathione + NADH | - |
? | |
S-formylglutathione + NADH | - |
Komagataella pastoris | formaldehyde + glutathione + NAD+ | - |
r | |
S-formylglutathione + NADH | - |
[Candida] boidinii | formaldehyde + glutathione + NAD+ | - |
r | |
S-formylglutathione + NADH | - |
[Candida] boidinii 2201 | formaldehyde + glutathione + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Komagataella pastoris |
dimer | 2 * 43000, SDS-PAGE | [Candida] boidinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Komagataella pastoris |
8 | - |
- |
[Candida] boidinii |
8 | - |
- |
Ogataea angusta |
8.5 | - |
- |
Komagataella pastoris |
8.5 | - |
- |
[Candida] boidinii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 20°C, 1 h, stable | [Candida] boidinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Komagataella pastoris | |
NAD+ | - |
[Candida] boidinii | |
NAD+ | - |
Ogataea angusta | |
NAD+ | - |
Pichia sp. | |
NADH | - |
Komagataella pastoris | |
NADH | - |
[Candida] boidinii | |
NADP+ | no activity with NADP+ | Komagataella pastoris | |
NADP+ | no activity with NADP+ | [Candida] boidinii |