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Literature summary for 1.1.1.282 extracted from

  • Singh, S.; Stavrinides, J.; Christendat, D.; Guttman, D.S.
    A phylogenomic analysis of the shikimate dehydrogenases reveals broadscale functional diversification and identifies one functionally distinct subclass (2008), Mol. Biol. Evol., 25, 2221-2232.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs) Pseudomonas putida KT2440

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.572
-
NAD+ in the presence of shikimate Pseudomonas putida KT2440
0.783
-
quinate in the presence of 2 mM NAD+ Pseudomonas putida KT2440
1.083
-
NAD+ in the presence of quinate Pseudomonas putida KT2440
4.2
-
shikimate in the presence of 2 mM NAD+ Pseudomonas putida KT2440

Organism

Organism UniProt Comment Textmining
Pseudomonas putida KT2440 Q88GF6
-
-
Pseudomonas putida KT2440 Q88JP1
-
-
Pseudomonas putida KT2440 Q88K85
-
-

Purification (Commentary)

Purification (Comment) Organism
by nickel-nitrilotriacetic acid affinity chromatography Pseudomonas putida KT2440

Storage Stability

Storage Stability Organism
4Ā°C, 10 mM Tris-HCl buffer, pH 7.5, 500 mM NaCl, 5% glycerol Pseudomonas putida KT2440
4Ā°C, 10 mM TrisĀ–HCl buffer, pH 7.5, 500 mM NaCl, 5% glycerol Pseudomonas putida KT2440

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-quinate + NAD+
-
Pseudomonas putida KT2440 3-dehydroquinate + NADH + H+
-
r
additional information broad extent to which the SDH enzyme superfamily has diversified. 5 evolutionarily distinct SDH homologs in the genome of the common soil-inhabiting bacterium, Pseudomonas putida KT2440 Pseudomonas putida KT2440 ?
-
?
quinate + NAD(P)+
-
Pseudomonas putida KT2440 3-dehydroquinate + NAD(P)H + H+
-
?
shikimate + NAD(P)+
-
Pseudomonas putida KT2440 3-dehydroshikimate + NAD(P)H + H+
-
?
shikimate + NAD+
-
Pseudomonas putida KT2440 3-dehydroshikimate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
rifI
-
Pseudomonas putida KT2440
SDH
-
Pseudomonas putida KT2440
sdhL
-
Pseudomonas putida KT2440
YdiB
-
Pseudomonas putida KT2440

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37.7
-
quinate in the presence of 2 mM NAD+ Pseudomonas putida KT2440
55.7
-
shikimate in the presence of 2 mM NAD+ Pseudomonas putida KT2440
55.7
-
NAD+ in the presence of quinate Pseudomonas putida KT2440
61.1
-
NAD+ in the presence of shikimate Pseudomonas putida KT2440

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Pseudomonas putida KT2440