Application | Comment | Organism |
---|---|---|
agriculture | development of novel herbicides | Escherichia coli |
medicine | development of novel antimicrobial agents | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D107A | site-directed mutagenesis | Escherichia coli |
K71G | site-directed mutagenesis | Escherichia coli |
N92A | site-directed mutagenesis | Escherichia coli |
Q262A | site-directed mutagenesis | Escherichia coli |
S22A | site-directed mutagenesis | Escherichia coli |
S67A | site-directed mutagenesis, increased activity compared to wild type enzyme | Escherichia coli |
T106A | site-directed mutagenesis | Escherichia coli |
Y39F | site-directed mutagenesis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
NAD+ | mutant K71A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.0017 | - |
shikimate | mutant S67A, 20°C, pH 9.0 | Escherichia coli | |
0.002 | - |
NAD+ | mutant K71A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.0028 | - |
shikimate | mutant Q262A, 20°C, pH 9.0 | Escherichia coli | |
0.0029 | - |
shikimate | wild type enzyme, 20°C, pH 9.0 | Escherichia coli | |
0.0032 | - |
shikimate | mutant S22A, 20°C, pH 9.0 | Escherichia coli | |
0.0032 | - |
shikimate | mutant Y39F, 20°C, pH 9.0 | Escherichia coli | |
0.0054 | - |
L-quinate | mutant Y39F, 20°C, pH 9.0 | Escherichia coli | |
0.0057 | - |
L-quinate | mutant S22A, 20°C, pH 9.0 | Escherichia coli | |
0.0068 | - |
NAD+ | mutant D107A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.0091 | - |
L-quinate | wild type enzyme, 20°C, pH 9.0 | Escherichia coli | |
0.0107 | - |
NAD+ | mutant Q262A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.0121 | - |
NAD+ | mutant S67A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.0122 | - |
NAD+ | wild type enzyme, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.0125 | - |
NAD+ | mutant Y39F, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.0126 | - |
NAD+ | mutant S67A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.0136 | - |
L-quinate | mutant Q262A, 20°C, pH 9.0 | Escherichia coli | |
0.0142 | - |
NAD+ | mutant Q262A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.0157 | - |
L-quinate | mutant S67A, 20°C, pH 9.0 | Escherichia coli | |
0.0158 | - |
NAD+ | mutant T106A, in the presence of shihikimat, 20°C, pH 9.0 | Escherichia coli | |
0.0158 | - |
NAD+ | mutant Y39F, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.0168 | - |
NAD+ | mutant S22A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.018 | - |
NAD+ | mutant S22A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.0184 | - |
NAD+ | wild type enzyme, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.0578 | - |
NAD+ | mutant T106A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.199 | - |
shikimate | mutant T106A, 20°C, pH 9.0 | Escherichia coli | |
0.513 | - |
shikimate | mutant K71A, 20°C, pH 9.0 | Escherichia coli | |
20.52 | - |
L-quinate | mutant T106A, 20°C, pH 9.0 | Escherichia coli | |
32.68 | - |
L-quinate | mutant K71A, 20°C, pH 9.0 | Escherichia coli | |
40.07 | - |
shikimate | mutant D107A, 20°C, pH 9.0 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31350 | - |
2 * 31350, wild type enzyme, gel filtration | Escherichia coli |
33000 | - |
SDS-PAGE, mutant N92A | Escherichia coli |
57000 | - |
SDS-PAGE, wild type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6D5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroquinate + NADPH + H+ | - |
Escherichia coli | L-quinate + NADP+ | - |
r | |
3-dehydroshikimate + NADH + H+ | - |
Escherichia coli | shikimate + NAD+ | - |
r | |
L-quinate + NADP+ | - |
Escherichia coli | 3-dehydroquinate + NADPH + H+ | - |
r | |
shikimate + NAD+ | - |
Escherichia coli | 3-dehydroshikimate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31350, wild type enzyme, gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
quinate/shikimate 5-dehydrogenase | - |
Escherichia coli |
YdiB | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NAD+ | mutant K71A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.011 | - |
shikimate | mutant K71A, 20°C, pH 9.0 | Escherichia coli | |
0.012 | - |
L-quinate | mutant K71A, 20°C, pH 9.0 | Escherichia coli | |
0.012 | - |
NAD+ | mutant K71A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.022 | - |
NAD+ | mutant D107A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.022 | - |
NAD+ | mutant Q262A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.023 | - |
NAD+ | mutant S67A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.024 | - |
L-quinate | mutant S67A, 20°C, pH 9.0 | Escherichia coli | |
0.043 | - |
NAD+ | mutant Q262A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.05 | - |
shikimate | mutant Q262A, 20°C, pH 9.0 | Escherichia coli | |
0.05 | - |
L-quinate | mutant Q262A, 20°C, pH 9.0 | Escherichia coli | |
0.054 | - |
shikimate | mutant T106A, 20°C, pH 9.0 | Escherichia coli | |
0.06 | - |
L-quinate | mutant Y39F, 20°C, pH 9.0 | Escherichia coli | |
0.068 | - |
L-quinate | mutant S22A, 20°C, pH 9.0 | Escherichia coli | |
0.082 | - |
NAD+ | mutant S22A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.09 | - |
NAD+ | mutant Y39F, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.091 | - |
shikimate | wild type enzyme, 20°C, pH 9.0 | Escherichia coli | |
0.105 | - |
NAD+ | wild type enzyme, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.108 | - |
NAD+ | mutant T106A, in the presence of shihikimat, 20°C, pH 9.0 | Escherichia coli | |
0.109 | - |
shikimate | mutant S22A, 20°C, pH 9.0 | Escherichia coli | |
0.113 | - |
L-quinate | wild type enzyme, 20°C, pH 9.0 | Escherichia coli | |
0.122 | - |
NAD+ | mutant T106A, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.132 | - |
shikimate | mutant D107A, 20°C, pH 9.0 | Escherichia coli | |
0.142 | - |
NAD+ | wild type enzyme, in the presence of L-quinate, 20°C, pH 9.0 | Escherichia coli | |
0.148 | - |
shikimate | mutant S67A, 20°C, pH 9.0 | Escherichia coli | |
0.153 | - |
NAD+ | mutant S22A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.178 | - |
NAD+ | mutant S67A, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli | |
0.187 | - |
shikimate | mutant Y39F, 20°C, pH 9.0 | Escherichia coli | |
0.233 | - |
L-quinate | mutant T106A, 20°C, pH 9.0 | Escherichia coli | |
0.24 | - |
NAD+ | mutant Y39F, in the presence of shikimate, 20°C, pH 9.0 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli | |
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |