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Literature summary for 1.1.1.28 extracted from

  • Wu, B.; Yu, Q.; Zheng, S.; Pedroso, M.; Guddat, L.; He, B.; Schenk, G.
    Relative catalytic efficiencies and transcript levels of three d- and two l-lactate dehydrogenases for optically pure d-lactate production in Sporolactobacillus inulinus (2019), MicrobiologyOpen, 8, e704 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sporolactobacillus inulinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 pyruvate isoform LDHD1, pH 6, 35°C Sporolactobacillus inulinus
1.4
-
 pyruvate isoform LDHD2, pH 7.5, 35°C Sporolactobacillus inulinus
3.1
-
 pyruvate isoform LDHD3, pH 5.5, 35°C Sporolactobacillus inulinus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ 5 mM, about 10% residual activity Sporolactobacillus inulinus

Organism

Organism UniProt Comment Textmining
Sporolactobacillus inulinus
-
-
-
Sporolactobacillus inulinus YBS1-5
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NADH + H+
-
 Sporolactobacillus inulinus 2-hydroxybutanoate + NAD+
-
 ?
2-oxovalerate + NADH + H+
-
 Sporolactobacillus inulinus 2-hydroxyvalerate + NAD+
-
 ?
glyoxylate + NADH + H+ best substrate of isoform LDHL1 Sporolactobacillus inulinus glycolate + NAD+
-
 ?
phenylpyruvate + NADH + H+
-
 Sporolactobacillus inulinus 2-hydroxy-3-phenylpropanoate + NAD+
-
 ?
pyruvate + NADH + H+
-
 Sporolactobacillus inulinus D-lactate + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 36600, isoform LDHD2, calculated from sequence and SDS-PAGE Sporolactobacillus inulinus
? x * 36700, isoform LDHD1, calculated from sequence and SDS-PAGE Sporolactobacillus inulinus
? x * 37200, isoform LDHD2, calculated from sequence and SDS-PAGE Sporolactobacillus inulinus

Synonyms

Synonyms Comment Organism
LDHD1
-
 Sporolactobacillus inulinus
LDHD2
-
 Sporolactobacillus inulinus
LDHD3
-
 Sporolactobacillus inulinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.7
-
 pyruvate isoform LDHD3, pH 5.5, 35°C Sporolactobacillus inulinus
44.7
-
 pyruvate isoform LDHD2, pH 7.5, 35°C Sporolactobacillus inulinus
75.8
-
 pyruvate isoform LDHD1, pH 6, 35°C Sporolactobacillus inulinus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5
-
 pyruvate isoform LDHD3, pH 5.5, 35°C Sporolactobacillus inulinus
32
-
 pyruvate isoform LDHD2, pH 7.5, 35°C Sporolactobacillus inulinus
161
-
 pyruvate isoform LDHD1, pH 6, 35°C Sporolactobacillus inulinus