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Literature summary for 1.1.1.28 extracted from
- Biophysical and biochemical characterization of tp0037, a D-lactate dehydrogenase, supports an acetogenic energy conservation pathway in Treponema pallidum (2020), mBio, 11, e02249 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to 1.38 A resolution. The structure features two domains with a cleft between them. The smaller catalytic domain comprises a central beta-sheet flanked by alpha-helices. The sheet is composed of five parallel strands and one antiparallel one. The larger nucleotide-binding domain harbors a centrally located seven-strand parallel beta-sheet | Treponema pallidum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H296A | loss of activity | Treponema pallidum |
| R235A | loss of activity | Treponema pallidum |
| Y101A | strong decrease in activity | Treponema pallidum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78100 | - |
dynamic light scattering | Treponema pallidum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Treponema pallidum | O83080 | - |
- |
| Treponema pallidum Nichols | O83080 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
mutant Y101A, substrate L-lactate, 37°C, pH not specified in the publication | Treponema pallidum |
| 7 | - |
wild-type, substrate L-lactate, 37°C, pH not specified in the publication | Treponema pallidum |
| 172 | - |
mutant Y101A, substrate D-lactate, 37°C, pH not specified in the publication | Treponema pallidum |
| 5000 | - |
wild-type, substrate D-lactate, 37°C, pH not specified in the publication | Treponema pallidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-lactate + NAD+ | - |
Treponema pallidum | pyruvate + NADH + H+ | - |
? |  |
| D-lactate + NAD+ | - |
Treponema pallidum Nichols | pyruvate + NADH + H+ | - |
? | |
| L-lactate + NAD+ | very poor substrate | Treponema pallidum | pyruvate + NADH + H+ | - |
? | |
| L-lactate + NAD+ | very poor substrate | Treponema pallidum Nichols | pyruvate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 39190, calculated from sequence | Treponema pallidum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tp0037 | - |
Treponema pallidum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48.7 | - |
mutant H296A, melting temperature | Treponema pallidum |
| 49.1 | - |
wild-type, melting temperature | Treponema pallidum |
| 49.3 | - |
wild-type, melting temperature, presence of 20 mM L-lactate | Treponema pallidum |
| 50.1 | - |
wild-type, melting temperature, presence of 20 mM D-lactate | Treponema pallidum |
| 53.4 | - |
wild-type, melting temperature, presence of 5 mM NAD+ | Treponema pallidum |
| 53.5 | - |
mutant Y101A, melting temperature | Treponema pallidum |
| 55.8 | - |
mutant R235A, melting temperature | Treponema pallidum |
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