Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.28 extracted from

  • Jia, B.; Pu, Z.J.; Tang, K.; Jia, X.; Kim, K.H.; Liu, X.; Jeon, C.O.
    Catalytic, computational, and evolutionary analysis of the (R)-lactate dehydrogenases responsible for D-lactic acid production in lactic acid bacteria (2018), J. Agric. Food Chem., 66, 8371-8381 .
    View publication on PubMed
Search for more literature for 1.1.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leuconostoc mesenteroides subsp. mesenteroides

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling and docking of substrates. D-LDH1 binds pyruvate using Tyr101, Arg235, and His296 by hydrogen bonds in the NADH-pyruvate-LDH1 complex Leuconostoc mesenteroides subsp. mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 NAD+ pH 9, 30°C Leuconostoc mesenteroides subsp. mesenteroides
0.32
-
 NADH pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides
2.66
-
 pyruvate pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides
78.8
-
 oxaloacetate pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides
87.9
-
 (R)-lactate pH 8, , 30°C Leuconostoc mesenteroides subsp. mesenteroides
234
-
 2-oxobutanoate pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides subsp. mesenteroides
-
-
-
Leuconostoc mesenteroides subsp. mesenteroides J18
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
 Leuconostoc mesenteroides subsp. mesenteroides pyruvate + NADH + H+
-
 r
(R)-lactate + NAD+
-
 Leuconostoc mesenteroides subsp. mesenteroides J18 pyruvate + NADH + H+
-
 r
2-oxobutanoate + NADH + H+
-
 Leuconostoc mesenteroides subsp. mesenteroides 2-hydroxybutanoate + NAD+
-
 ?
2-oxobutanoate + NADH + H+
-
 Leuconostoc mesenteroides subsp. mesenteroides J18 2-hydroxybutanoate + NAD+
-
 ?
additional information no substrate: phenylpyruvate Leuconostoc mesenteroides subsp. mesenteroides ?
-
-
additional information no substrate: phenylpyruvate Leuconostoc mesenteroides subsp. mesenteroides J18 ?
-
-
oxaloacetate + NADH + H+
-
 Leuconostoc mesenteroides subsp. mesenteroides D-malate + NAD+
-
 ?
oxaloacetate + NADH + H+
-
 Leuconostoc mesenteroides subsp. mesenteroides J18 D-malate + NAD+
-
 ?
pyruvate + NADH + H+
-
 Leuconostoc mesenteroides subsp. mesenteroides (R)-lactate + NAD+
-
 r
pyruvate + NADH + H+
-
 Leuconostoc mesenteroides subsp. mesenteroides J18 (R)-lactate + NAD+
-
 r

Synonyms

Synonyms Comment Organism
D-LDH1
-
 Leuconostoc mesenteroides subsp. mesenteroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
340
-
 NAD+ pH 9, 30°C Leuconostoc mesenteroides subsp. mesenteroides
910
-
 (R)-lactate pH 8, , 30°C Leuconostoc mesenteroides subsp. mesenteroides
1570
-
 oxaloacetate pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides
2220
-
 2-oxobutanoate pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides
2750
-
 pyruvate pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides
3260
-
 NADH pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 optimum of pyruvate reduction Leuconostoc mesenteroides subsp. mesenteroides
10
-
 optimum of lactate oxidation Leuconostoc mesenteroides subsp. mesenteroides

General Information

General Information Comment Organism
physiological function isoform D-LDH1 is the major enzyme responsible for D-lactic acid production in Leuconostoc mesenteroides J18 Leuconostoc mesenteroides subsp. mesenteroides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.48
-
 2-oxobutanoate pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides
10.32
-
 (R)-lactate pH 8, , 30°C Leuconostoc mesenteroides subsp. mesenteroides
19.87
-
 oxaloacetate pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides
1030
-
 pyruvate pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides
2820
-
 NAD+ pH 9, 30°C Leuconostoc mesenteroides subsp. mesenteroides
10200
-
 NADH pH 8, 30°C Leuconostoc mesenteroides subsp. mesenteroides