Crystallization (Comment) | Organism |
---|---|
homology modeling of structure | Pediococcus acidilactici |
Protein Variants | Comment | Organism |
---|---|---|
F298L | negligible activity | Pediococcus acidilactici |
F298V | negligible activity | Pediococcus acidilactici |
N76A | reduced activity compared to wild-type | Pediococcus acidilactici |
N76I | negligible activity | Pediococcus acidilactici |
N76L | reduced activity compared to wild-type | Pediococcus acidilactici |
N76V | reduced activity compared to wild-type | Pediococcus acidilactici |
V77I | reduced activity compared to wild-type | Pediococcus acidilactici |
Y100F | reduced activity compared to wild-type | Pediococcus acidilactici |
Y100I | negligible activity | Pediococcus acidilactici |
Y100L | negligible activity | Pediococcus acidilactici |
Y51A | mutant with improved catalytic efficiency on phenylpyruvate | Pediococcus acidilactici |
Y51F | mutant with improved catalytic efficiency on 2-oxobutanoate and 3-methyl-2-oxobutanoate of 37.2 and 23.2 per s and mM, respectively | Pediococcus acidilactici |
Y51L | mutant with improved catalytic efficiency on phenylpyruvate of 2200 per s and mM | Pediococcus acidilactici |
Y51M | mutant with improved catalytic efficiency on phenylpyruvate | Pediococcus acidilactici |
Y51S | mutant with improved catalytic efficiency on phenylpyruvate | Pediococcus acidilactici |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
phenylpyruvate | mutant Y51A, pH 5.5, 30°C | Pediococcus acidilactici | |
0.17 | - |
phenylpyruvate | mutant Y51L, pH 5.5, 30°C | Pediococcus acidilactici | |
0.22 | - |
phenylpyruvate | mutant Y51F, pH 5.5, 30°C | Pediococcus acidilactici | |
0.22 | - |
phenylpyruvate | mutant Y51M, pH 5.5, 30°C | Pediococcus acidilactici | |
0.4 | - |
phenylpyruvate | mutant Y51S, pH 5.5, 30°C | Pediococcus acidilactici | |
2.6 | - |
phenylpyruvate | wild-type, pH 5.5, 30°C | Pediococcus acidilactici | |
2.7 | - |
2-oxobutanoate | mutant Y51F, pH 5.5, 30°C | Pediococcus acidilactici | |
7.5 | - |
2-oxobutanoate | mutant Y51M, pH 5.5, 30°C | Pediococcus acidilactici | |
7.7 | - |
3-methyl-2-oxobutanoate | mutant Y51F, pH 5.5, 30°C | Pediococcus acidilactici | |
8.6 | - |
3-methyl-2-oxobutanoate | mutant Y51M, pH 5.5, 30°C | Pediococcus acidilactici | |
11 | - |
2-oxobutanoate | wild-type, pH 5.5, 30°C | Pediococcus acidilactici | |
12 | - |
2-oxobutanoate | mutant Y51L, pH 5.5, 30°C | Pediococcus acidilactici | |
12 | - |
3-methyl-2-oxobutanoate | mutant Y51L, pH 5.5, 30°C | Pediococcus acidilactici | |
38 | - |
3-methyl-2-oxobutanoate | wild-type, pH 5.5, 30°C | Pediococcus acidilactici |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pediococcus acidilactici | E0NDE9 | - |
- |
Pediococcus acidilactici DSM 20284 | E0NDE9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NADH + H+ | - |
Pediococcus acidilactici | 2-hydroxybutanoate + NAD+ | - |
? | |
2-oxobutanoate + NADH + H+ | - |
Pediococcus acidilactici DSM 20284 | 2-hydroxybutanoate + NAD+ | - |
? | |
3-methyl-2-oxobutanoate + NADH + H+ | - |
Pediococcus acidilactici | 2-hydroxy-3-methylbutanoate + NAD+ | - |
? | |
3-methyl-2-oxobutanoate + NADH + H+ | - |
Pediococcus acidilactici DSM 20284 | 2-hydroxy-3-methylbutanoate + NAD+ | - |
? | |
phenylpyruvate + NADH + H+ | - |
Pediococcus acidilactici | (R)-3-phenyllactate + NAD+ | - |
? | |
phenylpyruvate + NADH + H+ | - |
Pediococcus acidilactici DSM 20284 | (R)-3-phenyllactate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-LDH | - |
Pediococcus acidilactici |
ldhd | - |
Pediococcus acidilactici |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.9 | - |
3-methyl-2-oxobutanoate | wild-type, pH 5.5, 30°C | Pediococcus acidilactici | |
41.3 | - |
phenylpyruvate | wild-type, pH 5.5, 30°C | Pediococcus acidilactici | |
50.7 | - |
2-oxobutanoate | wild-type, pH 5.5, 30°C | Pediococcus acidilactici | |
76.2 | - |
3-methyl-2-oxobutanoate | mutant Y51M, pH 5.5, 30°C | Pediococcus acidilactici | |
90.2 | - |
3-methyl-2-oxobutanoate | mutant Y51L, pH 5.5, 30°C | Pediococcus acidilactici | |
99.4 | - |
2-oxobutanoate | mutant Y51F, pH 5.5, 30°C | Pediococcus acidilactici | |
152 | - |
2-oxobutanoate | mutant Y51M, pH 5.5, 30°C | Pediococcus acidilactici | |
179 | - |
3-methyl-2-oxobutanoate | mutant Y51F, pH 5.5, 30°C | Pediococcus acidilactici | |
199 | - |
2-oxobutanoate | mutant Y51L, pH 5.5, 30°C | Pediococcus acidilactici | |
239 | - |
phenylpyruvate | mutant Y51S, pH 5.5, 30°C | Pediococcus acidilactici | |
269 | - |
phenylpyruvate | mutant Y51A, pH 5.5, 30°C | Pediococcus acidilactici | |
376 | - |
phenylpyruvate | mutant Y51L, pH 5.5, 30°C | Pediococcus acidilactici | |
391 | - |
phenylpyruvate | mutant Y51F, pH 5.5, 30°C | Pediococcus acidilactici | |
462 | - |
phenylpyruvate | mutant Y51M, pH 5.5, 30°C | Pediococcus acidilactici |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | - |
3-methyl-2-oxobutanoate | wild-type, pH 5.5, 30°C | Pediococcus acidilactici | |
4.4 | - |
2-oxobutanoate | wild-type, pH 5.5, 30°C | Pediococcus acidilactici | |
6 | 12 | phenylpyruvate | mutant Y51S, pH 5.5, 30°C | Pediococcus acidilactici | |
7.4 | - |
3-methyl-2-oxobutanoate | mutant Y51L, pH 5.5, 30°C | Pediococcus acidilactici | |
8.9 | - |
3-methyl-2-oxobutanoate | mutant Y51M, pH 5.5, 30°C | Pediococcus acidilactici | |
15.9 | - |
phenylpyruvate | wild-type, pH 5.5, 30°C | Pediococcus acidilactici | |
16.8 | - |
2-oxobutanoate | mutant Y51L, pH 5.5, 30°C | Pediococcus acidilactici | |
20.7 | - |
2-oxobutanoate | mutant Y51M, pH 5.5, 30°C | Pediococcus acidilactici | |
23.2 | - |
3-methyl-2-oxobutanoate | mutant Y51F, pH 5.5, 30°C | Pediococcus acidilactici | |
37.2 | - |
2-oxobutanoate | mutant Y51F, pH 5.5, 30°C | Pediococcus acidilactici | |
1720 | - |
phenylpyruvate | mutant Y51A, pH 5.5, 30°C | Pediococcus acidilactici | |
1880 | - |
phenylpyruvate | mutant Y51F, pH 5.5, 30°C | Pediococcus acidilactici | |
2130 | - |
phenylpyruvate | mutant Y51M, pH 5.5, 30°C | Pediococcus acidilactici | |
2200 | - |
phenylpyruvate | mutant Y51L, pH 5.5, 30°C | Pediococcus acidilactici |