Literature summary for 1.1.1.28 extracted from

Singhvi, M.; Jadhav, A.; Gokhale, D.
Supplementation of medium with diammonium hydrogen phosphate enhanced the D-lactate dehydrogenase levels leading to increased D-lactic acid productivity (2013), Biores. Technol., 146, 736-739.

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Organism

Organism	UniProt	Comment	Textmining
Lactobacillus delbrueckii subsp. lactis	-	mutant strain RM2-24 derived from strain NCIM 2368, which is derived from strain ATCC 8000	-
Lactobacillus delbrueckii subsp. lactis DSM 20072	-	mutant strain RM2-24 derived from strain NCIM 2368, which is derived from strain ATCC 8000	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	42°C, addition of 5 g/l peptone and 1 g/l (NH4)2HPO4 enhance D-lactic acid production by 32%, as compared to that obtained from non supplemented media, with a productivity of 3.0 g/l/h. Lactate dehydrogenase (LDH) expression profile in the different media, and effects of various nitrogen sources on D-lactic acid production, overview	Lactobacillus delbrueckii subsp. lactis	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.15	0.25	in vivo activity, crude enzyme, cells grown with or without peptone and (NH4)2HPO4, pH 7.3, 42°C	Lactobacillus delbrueckii subsp. lactis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3	-	assay at	Lactobacillus delbrueckii subsp. lactis

Expression

Organism	Comment	Expression
Lactobacillus delbrueckii subsp. lactis	supplementation of the medium with diammonium hydrogen phosphate enhances the D-lactate dehydrogenase levels leading to increased D-lactic acid productivity	up

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Release 2023.1 (January 2023)