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Literature summary for 1.1.1.28 extracted from

  • Furukawa, N.; Miyanaga, A.; Togawa, M.; Nakajima, M.; Taguchi, H.
    Diverse allosteric and catalytic functions of tetrameric D-lactate dehydrogenases from three Gram-negative bacteria (2014), AMB Express, 4, 76.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
fructose 1,6-bisphosphate required Fusobacterium nucleatum subsp. nucleatum
fructose 1,6-bisphosphate required Pseudomonas aeruginosa
additional information no activation of the enzyme by fructose 1,6-bisphosphate and Mg2+ Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene ecd_01352, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) Escherichia coli
gene fn0511, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) Fusobacterium nucleatum subsp. nucleatum
gene pa0927, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
glyoxylate
-
Pseudomonas aeruginosa
Hydroxypyruvate
-
Pseudomonas aeruginosa
oxaloacetate
-
Escherichia coli
oxaloacetate
-
Fusobacterium nucleatum subsp. nucleatum
oxamate constant inhibition at pH 7.0 and pH 8.0 Escherichia coli
oxamate inhibits the catalytic reaction of FNLDH competitively with pyruvate, but markedly enhances the enzyme reaction at low concentration through canceling of the apparent homotropic cooperativity at pH 8.0 Fusobacterium nucleatum subsp. nucleatum
oxamate inhibits the catalytic reaction of PALDH in a mixed--type manner at pH 7.0, but markedly enhances the enzyme reaction at low concentration through canceling of the apparent homotropic cooperativity at pH 8.0 Pseudomonas aeruginosa
phenylpyruvate
-
Fusobacterium nucleatum subsp. nucleatum
pyruvate
-
Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows negative cooperativity in the pyruvate saturation curve above pH 7.5 Pseudomonas aeruginosa
additional information
-
additional information kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5 Fusobacterium nucleatum subsp. nucleatum
additional information
-
additional information kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5 Escherichia coli
0.019
-
NADH pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
0.022
-
NADH pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
0.028
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
0.031
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
0.035
-
NADH pH 8.0, 30°C, recombinant enzyme, with or without fructose 1,6-bisphosphate and Mg2+ Pseudomonas aeruginosa
0.036
-
NADH pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
0.055
-
NADH pH 7.0, 30°C, recombinant enzyme Escherichia coli
0.088
-
NADH pH 8.0, 30°C, recombinant enzyme Escherichia coli
0.09
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
0.092
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
0.1
-
pyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
0.31
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
0.34
-
pyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
0.36
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
0.64
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
0.68
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
0.85
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Pseudomonas aeruginosa
1.2
-
pyruvate pH 8.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.2
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
1.7
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1.7
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
2.5
-
pyruvate pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
2.5
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
2.6
-
pyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
2.6
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
3
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
3.5
-
2-oxoisovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
3.8
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
4
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
5.8
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
6.5
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
6.8
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
7.1
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
7.2
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
7.6
-
pyruvate pH 8.0, 30°C, recombinant enzyme Escherichia coli
11
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
12
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
12
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
18
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
18
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
20
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Escherichia coli
30
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Escherichia coli
54
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Fusobacterium nucleatum subsp. nucleatum
Mg2+ activates Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37800
-
-
Pseudomonas aeruginosa
38500
-
-
Escherichia coli
40000
-
4 * 40000, about, sequence calculation Fusobacterium nucleatum subsp. nucleatum
120000
-
recombinant His-tagged enzyme, gel filtration Pseudomonas aeruginosa
150000
-
recombinant His-tagged enzyme, peak 2, gel filtration Escherichia coli
160000
-
recombinant His-tagged enzyme, gel filtration Fusobacterium nucleatum subsp. nucleatum
330000
-
recombinant His-tagged enzyme, peak 1, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + NAD+ Fusobacterium nucleatum subsp. nucleatum
-
pyruvate + NADH + H+
-
r
(R)-lactate + NAD+ Escherichia coli
-
pyruvate + NADH + H+
-
r
pyruvate + NADH + H+ Pseudomonas aeruginosa
-
(R)-lactate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A140N893
-
-
Fusobacterium nucleatum subsp. nucleatum Q8RG11 strain JCM 8532
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 Q8RG11 strain JCM 8532
-
Pseudomonas aeruginosa Q9I530
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration Fusobacterium nucleatum subsp. nucleatum
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration Pseudomonas aeruginosa
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
(R)-lactate + NAD+ = pyruvate + NADH + H+ allosteric mechanism Fusobacterium nucleatum subsp. nucleatum
(R)-lactate + NAD+ = pyruvate + NADH + H+ allosteric mechanism Pseudomonas aeruginosa
(R)-lactate + NAD+ = pyruvate + NADH + H+ allosteric mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
Fusobacterium nucleatum subsp. nucleatum pyruvate + NADH + H+
-
r
(R)-lactate + NAD+
-
Escherichia coli pyruvate + NADH + H+
-
r
2-oxobutyrate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Pseudomonas aeruginosa 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Escherichia coli 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 2-hydroxybutyrate + NAD+
-
r
2-oxocaproate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxycaproate + NAD+
-
r
2-oxocaproate + NADH + H+
-
Pseudomonas aeruginosa 2-hydroxycaproate + NAD+
-
r
2-oxocaproate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 2-hydroxycaproate + NAD+
-
r
2-oxoisocaproate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxyisocaproate + NAD+
-
r
2-oxoisocaproate + NADH + H+
-
Pseudomonas aeruginosa 2-hydroxyisocaproate + NAD+
-
r
2-oxoisovalerate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxyisovalerate + NAD+
-
r
2-oxovalerate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum 2-hydroxyvalerate + NAD+
-
r
2-oxovalerate + NADH + H+
-
Pseudomonas aeruginosa 2-hydroxyvalerate + NAD+
-
r
glyoxylate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum hydroxyacetate + NAD+
-
r
glyoxylate + NADH + H+
-
Pseudomonas aeruginosa hydroxyacetate + NAD+
-
r
glyoxylate + NADH + H+
-
Escherichia coli hydroxyacetate + NAD+
-
r
hydroxypyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum glycerate + NAD+
-
r
hydroxypyruvate + NADH + H+
-
Pseudomonas aeruginosa glycerate + NAD+
-
r
hydroxypyruvate + NADH + H+
-
Escherichia coli glycerate + NAD+
-
r
hydroxypyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 glycerate + NAD+
-
r
additional information the enzyme ECLDH exhibits high substrate specificity toward pyruvate. No or poor activity with 2-oxovalerate, 2-oxoisovalerate, 2-oxocaproate, 2-oxoisocaproate, phenylpyruvate, and hydroxyphenylpyruvate Escherichia coli ?
-
?
additional information the enzyme FNLDH exhibits a broad substrate specificity toward hydrophobic 2-oxoacids such as 2-oxobutyrate and 2-oxovalerate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate Fusobacterium nucleatum subsp. nucleatum ?
-
?
additional information the enzyme PALDH exhibits high substrate specificity toward pyruvate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate Pseudomonas aeruginosa ?
-
?
additional information the enzyme FNLDH exhibits a broad substrate specificity toward hydrophobic 2-oxoacids such as 2-oxobutyrate and 2-oxovalerate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 ?
-
?
oxaloacetate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Pseudomonas aeruginosa malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Escherichia coli malate + NAD+
-
r
phenylpyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum D-phenyllactate + NAD+
-
r
phenylpyruvate + NADH + H+
-
Pseudomonas aeruginosa D-phenyllactate + NAD+
-
r
pyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum (R)-lactate + NAD+
-
r
pyruvate + NADH + H+
-
Pseudomonas aeruginosa (R)-lactate + NAD+
-
r
pyruvate + NADH + H+
-
Escherichia coli (R)-lactate + NAD+
-
r
pyruvate + NADH + H+
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 (R)-lactate + NAD+
-
r

Subunits

Subunits Comment Organism
homooctamer 8 * 38500, about, sequence calculation, the apparently octameric form of ECLDH is likely an artificial product due to the overexpression, and exhibits slightly but significantly lower specific activity than the tetrameric form Escherichia coli
homotetramer 4 * 40000, about, sequence calculation Fusobacterium nucleatum subsp. nucleatum
homotetramer 4 * 38500, about, sequence calculation Escherichia coli
homotrimer 4 * 37800, about, sequence calculation Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
D-LDH
-
Fusobacterium nucleatum subsp. nucleatum
D-LDH
-
Pseudomonas aeruginosa
D-LDH
-
Escherichia coli
ECBD_2243
-
Escherichia coli
ECLDH
-
Escherichia coli
FN0511
-
Fusobacterium nucleatum subsp. nucleatum
FNLDH
-
Fusobacterium nucleatum subsp. nucleatum
PA0927
-
Pseudomonas aeruginosa
PALDH
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Fusobacterium nucleatum subsp. nucleatum
30
-
assay at Pseudomonas aeruginosa
30
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
39
-
purified recombinant His-tagged enzyme, pH 8.0, stable up to Fusobacterium nucleatum subsp. nucleatum
49
-
purified recombinant His-tagged enzyme, pH 8.0, stable up to Escherichia coli
57
-
purified recombinant His-tagged enzyme, pH 5.0, stable up to Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
18
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
31
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Escherichia coli
39
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
50
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
69
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
80
-
pyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
80
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
80
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
80
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
87
-
pyruvate pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
90
-
NADH pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
98
-
NADH pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
100
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Escherichia coli
100
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
100
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
110
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
120
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
120
-
2-oxoisovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
140
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
140
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
160
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
160
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
200
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
230
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
240
-
pyruvate pH 8.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
240
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Pseudomonas aeruginosa
270
-
NADH pH 8.0, 30°C, recombinant enzyme, with or without fructose 1,6-bisphosphate and Mg2+ Pseudomonas aeruginosa
290
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
310
-
NADH pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
370
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
380
-
pyruvate pH 8.0, 30°C, recombinant enzyme Escherichia coli
400
-
pyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
400
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
410
-
pyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
410
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
420
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
640
-
NADH pH 7.0, 30°C, recombinant enzyme Escherichia coli
690
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
710
-
NADH pH 8.0, 30°C, recombinant enzyme Escherichia coli
730
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
880
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
1100
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 assay at Fusobacterium nucleatum subsp. nucleatum
7 8 assay at Pseudomonas aeruginosa
7 8 assay at Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 purified recombinant His-tagged enzyme, 30°C, 1 h, stable at Pseudomonas aeruginosa
5 10 purified recombinant His-tagged enzyme, 30°C, 1 h, stable at Escherichia coli
5 8.5 purified recombinant His-tagged enzyme, 30°C, 1 h, stable at Fusobacterium nucleatum subsp. nucleatum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Fusobacterium nucleatum subsp. nucleatum
NAD+
-
Pseudomonas aeruginosa
NAD+
-
Escherichia coli
NADH
-
Fusobacterium nucleatum subsp. nucleatum
NADH
-
Pseudomonas aeruginosa
NADH
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Fusobacterium nucleatum subsp. nucleatum
additional information
-
additional information inhibition kinetics Pseudomonas aeruginosa
additional information
-
additional information inhibition kinetics Escherichia coli
1.8
-
pyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
5
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Escherichia coli
19
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
20
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
23
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
44
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
140
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum

General Information

General Information Comment Organism
evolution although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview Fusobacterium nucleatum subsp. nucleatum
evolution although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview Pseudomonas aeruginosa
evolution although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview Escherichia coli
metabolism the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions Fusobacterium nucleatum subsp. nucleatum
metabolism the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions Pseudomonas aeruginosa
metabolism the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
1
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Escherichia coli
2.6
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
3.2
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
4.2
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
5.3
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Escherichia coli
8.7
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
11
-
phenylpyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
18
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
21
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Escherichia coli
27
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
31
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
35
-
pyruvate pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
35
-
2-oxoisovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
50
-
pyruvate pH 8.0, 30°C, recombinant enzyme Escherichia coli
51
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
53
-
2-oxoisocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
60
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
61
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
65
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
87
-
2-oxocaproate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
130
-
glyoxylate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
140
-
Hydroxypyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
150
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
160
-
pyruvate pH 7.0, 30°C, recombinant enzyme Escherichia coli
200
-
pyruvate pH 8.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
200
-
2-oxovalerate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
230
-
pyruvate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
230
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
280
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Pseudomonas aeruginosa
440
-
2-oxobutyrate pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
820
-
pyruvate pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
2500
-
NADH pH 8.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
2800
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Fusobacterium nucleatum subsp. nucleatum
3700
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Fusobacterium nucleatum subsp. nucleatum
4000
-
pyruvate pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
4400
-
NADH pH 7.0, 30°C, recombinant enzyme Fusobacterium nucleatum subsp. nucleatum
7700
-
NADH pH 8.0, 30°C, recombinant enzyme Pseudomonas aeruginosa
7700
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Pseudomonas aeruginosa
7700
-
NADH pH 8.0, 30°C, recombinant enzyme, with Mg2+ Escherichia coli
7800
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Pseudomonas aeruginosa
8000
-
NADH pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate Escherichia coli
8100
-
NADH pH 8.0, 30°C, recombinant enzyme Escherichia coli
12000
-
NADH pH 7.0, 30°C, recombinant enzyme Escherichia coli
17000
-
NADH pH 7.0, 30°C, recombinant enzyme Pseudomonas aeruginosa