Literature summary for 1.1.1.28 extracted from

Shibahara, T.; Satomura, T.; Kawakami, R.; Ohshima, T.; Sakuraba, H.
Crystallization and preliminary X-ray analysis of a dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix (2011), Acta Crystallogr. Sect. F, 67, 1425-1427.

Cloned(Commentary)

Cloned (Comment)	Organism
gene APE_0487, expression in Escherichia coli strain Rosetta-gami 2 (DE3)	Aeropyrum pernix

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml of 15 mg/ml protein in 10 mM potassium phosphate, pH 7.0, with 0.002 ml of reservoir solution containing 14.4% PEG 8000, 80 mM cacodylate pH 6.5, 160 mM calcium acetate and 20% glycerol, and equilibration against 0.1 ml of reservoir solution, 25°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution	Aeropyrum pernix

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml of 15 mg/ml protein in 10 mM potassium phosphate, pH 7.0, with 0.002 ml of reservoir solution containing 14.4% PEG 8000, 80 mM cacodylate pH 6.5, 160 mM calcium acetate and 20% glycerol, and equilibration against 0.1 ml of reservoir solution, 25°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution

Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
59000	-	1 * 59000, recombinant enzyme, SDS-PAGE	Aeropyrum pernix
61000	-	recombinant enzyme, gel filtration	Aeropyrum pernix

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R)-lactate + NAD+	Aeropyrum pernix	-	pyruvate + NADH + H+	-	r
(R)-lactate + NAD+	Aeropyrum pernix DSM 11879	-	pyruvate + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Aeropyrum pernix	Q9YEU4	gene APE_0487; gene APE_0487	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain Rosetta-gami 2 (DE3) by nickel affnity chromatography and gel filtration	Aeropyrum pernix

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-lactate + NAD+	-	Aeropyrum pernix	pyruvate + NADH + H+	-	r
(R)-lactate + NAD+	-	Aeropyrum pernix DSM 11879	pyruvate + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
monomer	1 * 59000, recombinant enzyme, SDS-PAGE	Aeropyrum pernix

Synonyms

Synonyms	Comment	Organism
DLDH	-	Aeropyrum pernix

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Aeropyrum pernix
NADH	-	Aeropyrum pernix