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Literature summary for 1.1.1.28 extracted from

  • Shinoda, T.; Arai, K.; Shigematsu-Iida, M.; Ishikura, Y.; Tanaka, S.; Yamada, T.; Kimber, M.S.; Pai, E.F.; Fushinobu, S.; Taguchi, H.
    Distinct conformation-mediated functions of an active site loop in the catalytic reactions of NAD-dependent D-lactate dehydrogenase and formate dehydrogenase (2005), J. Biol. Chem., 280, 17068-17075.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method Lactiplantibacillus pentosus

Inhibitors

Inhibitors Comment Organism Structure
formate
-
Lactiplantibacillus pentosus
oxamate
-
Lactiplantibacillus pentosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
pyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
0.28
-
Hydroxypyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
5.4
-
glyoxylate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
8.1
-
2-oxobutyrate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
8.2
-
pyruvate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus
29
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus
58
-
2-oxobutyrate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus
72
-
glyoxylate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus pentosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + NADH + H+
-
Lactiplantibacillus pentosus 2-hydroxybutyrate + NAD+
-
?
glyoxylate + NADH + H+
-
Lactiplantibacillus pentosus hydroxyacetic acid + NAD+
-
?
hydroxypyruvate + NADH + H+
-
Lactiplantibacillus pentosus glycerate + NAD+
-
?
pyruvate + NADH + H+
-
Lactiplantibacillus pentosus (R)-lactate + NAD+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5
-
2-oxobutyrate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus
38
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus
50
-
glyoxylate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus
120
-
2-oxobutyrate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
260
-
Hydroxypyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
270
-
pyruvate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus
270
-
glyoxylate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
320
-
pyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8
-
oxamate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
43
-
oxamate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus
44
-
formate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
140
-
formate 30°C, pH 5.5, mutant enzyme N97D Lactiplantibacillus pentosus