Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method | Lactiplantibacillus pentosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
formate | - |
Lactiplantibacillus pentosus | |
oxamate | - |
Lactiplantibacillus pentosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
pyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
0.28 | - |
Hydroxypyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
5.4 | - |
glyoxylate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
8.1 | - |
2-oxobutyrate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
8.2 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus | |
29 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus | |
58 | - |
2-oxobutyrate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus | |
72 | - |
glyoxylate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus pentosus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + NADH + H+ | - |
Lactiplantibacillus pentosus | 2-hydroxybutyrate + NAD+ | - |
? | |
glyoxylate + NADH + H+ | - |
Lactiplantibacillus pentosus | hydroxyacetic acid + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | - |
Lactiplantibacillus pentosus | glycerate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Lactiplantibacillus pentosus | (R)-lactate + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
2-oxobutyrate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus | |
38 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus | |
50 | - |
glyoxylate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus | |
120 | - |
2-oxobutyrate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
260 | - |
Hydroxypyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
270 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus | |
270 | - |
glyoxylate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
320 | - |
pyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
oxamate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
43 | - |
oxamate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus | |
44 | - |
formate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
140 | - |
formate | 30°C, pH 5.5, mutant enzyme N97D | Lactiplantibacillus pentosus |