Protein Variants | Comment | Organism |
---|---|---|
F299G | mutation reduces enzyme activity with less marked change in substrate preference | Lactiplantibacillus pentosus |
F299S | mutation reduces enzyme activity with less marked change in substrate preference | Lactiplantibacillus pentosus |
Y52A | mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate | Lactiplantibacillus pentosus |
Y52D | mutation severely reduces enzyme activity | Lactiplantibacillus pentosus |
Y52R | mutation severely reduces enzyme activity, preference for large aliphatic 2-ketoacids and phenylpyruvate | Lactiplantibacillus pentosus |
Y52R/F299G | mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate and oxaloacetate, weak activity with 2-ketocaproate, 2-ketoglutarate and phenylpyruvate | Lactiplantibacillus pentosus |
Y52T | mutation severely reduces enzyme activity | Lactiplantibacillus pentosus |
Y52T/F299S | mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate, 2-ketoglutarate and oxaloacetate, weak activity with hydroxypyruvate and phenylpyruvate | Lactiplantibacillus pentosus |
Y52V | mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate | Lactiplantibacillus pentosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
0.06 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
0.12 | - |
pyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
0.124 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
0.14 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
0.15 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
0.28 | - |
Hydroxypyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
0.38 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
0.7 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
0.8 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
0.8 | - |
phenylpyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.18 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
1.8 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
2.1 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
2.1 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
2.2 | - |
oxaloacetate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
3 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
3.5 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
3.8 | - |
oxaloacetate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
5.5 | - |
2-ketoisovalerate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
6.9 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
7.2 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
8.1 | - |
2-Ketobutyrate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
8.7 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52T/D299S | Lactiplantibacillus pentosus | |
8.7 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
11.7 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
12.1 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
12.6 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
13.1 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
13.3 | - |
2-ketocaproate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
14.1 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
15.5 | - |
2-Ketoglutarate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
17 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
17 | - |
2-ketovalerate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
18.6 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
18.6 | - |
2-ketoisovalerate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
20.2 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
20.8 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
22 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
31 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
31 | - |
2-ketoisocaproate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
43.1 | - |
oxaloacetate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
51 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
53 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
102 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
189 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus pentosus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ketocaproate + NADH | - |
Lactiplantibacillus pentosus | 2-hydroxycaproate + NAD+ | - |
? | |
2-ketoisocaproate + NADH | - |
Lactiplantibacillus pentosus | 2-hydroxyisocaproate + NAD+ | - |
? | |
2-ketoisovalerate + NADH | - |
Lactiplantibacillus pentosus | 2-hydroxyisovalerate + NAD+ | - |
? | |
2-ketovalerate + NADH | - |
Lactiplantibacillus pentosus | 2-hydroxyvalerate + NAD+ | - |
? | |
2-oxobutyrate + NADH + H+ | - |
Lactiplantibacillus pentosus | 2-hydroxybutyrate + NAD+ | - |
? | |
2-oxoglutarate + NADH + H+ | - |
Lactiplantibacillus pentosus | 2-hydroxypentanedioate + NAD+ | - |
? | |
hydroxypyruvate + NADH | - |
Lactiplantibacillus pentosus | glycerate + NAD+ | - |
? | |
oxaloacetate + NADH | - |
Lactiplantibacillus pentosus | malate + NAD+ | - |
? | |
phenylpyruvate + NADH | - |
Lactiplantibacillus pentosus | phenyllactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Lactiplantibacillus pentosus | (R)-lactate + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
0.3 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
0.31 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
0.36 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
0.48 | - |
oxaloacetate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
0.51 | - |
2-Ketoglutarate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
0.7 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
2.1 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
2.8 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
3.4 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
5.2 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52T/D299S | Lactiplantibacillus pentosus | |
5.7 | - |
2-ketovalerate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
6.5 | - |
oxaloacetate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
8.1 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
11.1 | - |
2-ketoisocaproate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
11.7 | - |
2-ketoisovalerate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
13.8 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
15.8 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
15.9 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
17 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
19 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
21 | - |
2-ketocaproate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
22 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
25.5 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
27 | - |
2-ketoisovalerate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
35.8 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
37 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
40 | - |
phenylpyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
41 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
41.5 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
50 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
55.7 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
65.7 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
102 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
104 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
118 | - |
2-Ketobutyrate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
171 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
175 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
213 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
232 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
257 | - |
Hydroxypyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
280 | - |
oxaloacetate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
321 | - |
pyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
334 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
407 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
778 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Lactiplantibacillus pentosus |