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Literature summary for 1.1.1.28 extracted from

  • Ishikura, Y.; Tsuzuki, S.; Takahashi, O.; Tokuda, C.; Nakanishi, R.; Shinoda, T.; Taguchi, H.
    Recognition site for the side chain of 2-ketoacid substrate in D-lactate dehydrogenase (2005), J. Biochem., 138, 741-749.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
F299G mutation reduces enzyme activity with less marked change in substrate preference Lactiplantibacillus pentosus
F299S mutation reduces enzyme activity with less marked change in substrate preference Lactiplantibacillus pentosus
Y52A mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate Lactiplantibacillus pentosus
Y52D mutation severely reduces enzyme activity Lactiplantibacillus pentosus
Y52R mutation severely reduces enzyme activity, preference for large aliphatic 2-ketoacids and phenylpyruvate Lactiplantibacillus pentosus
Y52R/F299G mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate and oxaloacetate, weak activity with 2-ketocaproate, 2-ketoglutarate and phenylpyruvate Lactiplantibacillus pentosus
Y52T mutation severely reduces enzyme activity Lactiplantibacillus pentosus
Y52T/F299S mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate, 2-ketoglutarate and oxaloacetate, weak activity with hydroxypyruvate and phenylpyruvate Lactiplantibacillus pentosus
Y52V mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate Lactiplantibacillus pentosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
0.06
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
0.12
-
pyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
0.124
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
0.14
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
0.15
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
0.28
-
Hydroxypyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
0.38
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
0.7
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
0.8
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
0.8
-
phenylpyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.18
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
1.8
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
2.1
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
2.1
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
2.2
-
oxaloacetate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
3
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
3.5
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
3.8
-
oxaloacetate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
5.5
-
2-ketoisovalerate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
6.9
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
7.2
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
8.1
-
2-Ketobutyrate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
8.7
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52T/D299S Lactiplantibacillus pentosus
8.7
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
11.7
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
12.1
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
12.6
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
13.1
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
13.3
-
2-ketocaproate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
14.1
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
15.5
-
2-Ketoglutarate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
17
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
17
-
2-ketovalerate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
18.6
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
18.6
-
2-ketoisovalerate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
20.2
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
20.8
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
22
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
31
-
pyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
31
-
2-ketoisocaproate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
43.1
-
oxaloacetate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
51
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
53
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
102
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
189
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus pentosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ketocaproate + NADH
-
Lactiplantibacillus pentosus 2-hydroxycaproate + NAD+
-
?
2-ketoisocaproate + NADH
-
Lactiplantibacillus pentosus 2-hydroxyisocaproate + NAD+
-
?
2-ketoisovalerate + NADH
-
Lactiplantibacillus pentosus 2-hydroxyisovalerate + NAD+
-
?
2-ketovalerate + NADH
-
Lactiplantibacillus pentosus 2-hydroxyvalerate + NAD+
-
?
2-oxobutyrate + NADH + H+
-
Lactiplantibacillus pentosus 2-hydroxybutyrate + NAD+
-
?
2-oxoglutarate + NADH + H+
-
Lactiplantibacillus pentosus 2-hydroxypentanedioate + NAD+
-
?
hydroxypyruvate + NADH
-
Lactiplantibacillus pentosus glycerate + NAD+
-
?
oxaloacetate + NADH
-
Lactiplantibacillus pentosus malate + NAD+
-
?
phenylpyruvate + NADH
-
Lactiplantibacillus pentosus phenyllactate + NAD+
-
?
pyruvate + NADH + H+
-
Lactiplantibacillus pentosus (R)-lactate + NAD+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.056
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
0.3
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
0.31
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
0.36
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
0.48
-
oxaloacetate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
0.51
-
2-Ketoglutarate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
0.7
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
2.1
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
2.8
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
3.4
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
5.2
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52T/D299S Lactiplantibacillus pentosus
5.7
-
2-ketovalerate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
6.5
-
oxaloacetate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
8.1
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
11.1
-
2-ketoisocaproate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
11.7
-
2-ketoisovalerate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
13.8
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
15.8
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
15.9
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
17
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
19
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
21
-
2-ketocaproate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
22
-
pyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
25.5
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
27
-
2-ketoisovalerate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
35.8
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
37
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
40
-
phenylpyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
41
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
41.5
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
50
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
55.7
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
65.7
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
102
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
104
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
118
-
2-Ketobutyrate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
171
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
175
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
213
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
232
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
257
-
Hydroxypyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
280
-
oxaloacetate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
321
-
pyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
334
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
407
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
778
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Lactiplantibacillus pentosus