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Literature summary for 1.1.1.28 extracted from

  • Kochhar, S.; Chuard, N.; Hottinger, H.
    Glutamate 264 modulates the pH dependence of the NAD(+)-dependent D-lactate dehydrogenase (1992), J. Biol. Chem., 267, 20298-20301.
    View publication on PubMed
Search for more literature for 1.1.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lactobacillus delbrueckii subsp. bulgaricus

Protein Variants

Protein Variants Comment Organism
E264G shift of 2 units in optimal pH toward the acidic range Lactobacillus delbrueckii subsp. bulgaricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 pyruvate
-
 Lactobacillus delbrueckii subsp. bulgaricus
7
-
 oxaloacetate
-
 Lactobacillus delbrueckii subsp. bulgaricus
10
-
 Bromopyruvate
-
 Lactobacillus delbrueckii subsp. bulgaricus
100
-
 glyoxylate
-
 Lactobacillus delbrueckii subsp. bulgaricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + NAD+ Lactobacillus delbrueckii subsp. bulgaricus
-
 pyruvate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Lactobacillus delbrueckii subsp. bulgaricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactobacillus delbrueckii subsp. bulgaricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
 Lactobacillus delbrueckii subsp. bulgaricus pyruvate + NADH
-
 ?
bromopyruvate + NADH
-
 Lactobacillus delbrueckii subsp. bulgaricus 3-bromo-2-hydroxypropanoate + NAD+
-
 ?
glyoxylate + NADH
-
 Lactobacillus delbrueckii subsp. bulgaricus hydroxyacetic acid + NAD+
-
 ?
oxaloacetate + NADH
-
 Lactobacillus delbrueckii subsp. bulgaricus malate + NAD+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Lactobacillus delbrueckii subsp. bulgaricus