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Literature summary for 1.1.1.28 extracted from
- Cloning and overexpression of the Lactobacillus bulgaricus NAD(+)-dependent D-lactate dehydrogenase gene in Escherichia coli: purification and characterization of the recombinant enzyme (1992), Biochem. Biophys. Res. Commun., 185, 705-712.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lactobacillus delbrueckii subsp. bulgaricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | 1.6 | pyruvate | recombinant and wild-type enzyme | Lactobacillus delbrueckii subsp. bulgaricus |  |
| 7.5 | 9 | oxaloacetate | recombinant and wild-type enzyme | Lactobacillus delbrueckii subsp. bulgaricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, SDS-PAGE | Lactobacillus delbrueckii subsp. bulgaricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + NAD+ | Lactobacillus delbrueckii subsp. bulgaricus | - |
pyruvate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus delbrueckii subsp. bulgaricus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus delbrueckii subsp. bulgaricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1950 | 2100 | - |
Lactobacillus delbrueckii subsp. bulgaricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + NAD+ | - |
Lactobacillus delbrueckii subsp. bulgaricus | pyruvate + NADH | - |
? | |
| oxaloacetate + NADH | - |
Lactobacillus delbrueckii subsp. bulgaricus | malate + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 37000, SDS-PAGE | Lactobacillus delbrueckii subsp. bulgaricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Lactobacillus delbrueckii subsp. bulgaricus |
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