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Literature summary for 1.1.1.28 extracted from
- D-Lactate dehydrogenase from the horseshoe crab (1975), Methods Enzymol., 41B, 313-323.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol and EDTA stabilize | Limulus polyphemus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Limulus polyphemus |  |
| iodoacetate | - |
Limulus polyphemus | |
| oxamate | - |
Limulus polyphemus | |
| p-hydroxymercuribenzoate | - |
Limulus polyphemus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00021 | - |
NADH | - |
Limulus polyphemus | |
| 0.00044 | - |
NAD+ | - |
Limulus polyphemus | |
| 0.07 | - |
pyruvate | - |
Limulus polyphemus | |
| 3.8 | - |
(R)-lactate | - |
Limulus polyphemus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, gel filtration | Limulus polyphemus |
| 70000 | 80000 | gel filtration, equilibrium sedimentation | Limulus polyphemus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + NAD+ | Limulus polyphemus | - |
pyruvate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limulus polyphemus | - |
horseshoe crab | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Limulus polyphemus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Limulus polyphemus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + NAD+ | - |
Limulus polyphemus | pyruvate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, gel filtration | Limulus polyphemus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
3 min, 5% loss of activity | Limulus polyphemus |
| 60 | - |
3 min, 90% loss of activity | Limulus polyphemus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 633 | - |
NADH | - |
Limulus polyphemus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Limulus polyphemus | |
| NADH | - |
Limulus polyphemus | |
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Release 2023.1 (January 2023)
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