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Literature summary for 1.1.1.278 extracted from

  • Warneck, H.M.; Seitz, H.U.
    3beta-Hydroxysteroid oxidoreductase in suspension cultures of Digitalis lanata EHRH (1990), Z. Naturforsch. C, 45, 963-972.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DTT 1 mM, 1.75fold increase in activity Digitalis lanata

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol activates at 1 mM, inhibitory at 10 mM Digitalis lanata
ascorbate
-
Digitalis lanata
Ca2+
-
Digitalis lanata
Co2+
-
Digitalis lanata
L-cysteine 1 mM, together with 2-mercaptoethanl, up to 50% inhibition Digitalis lanata
Mg2+
-
Digitalis lanata
Mn2+
-
Digitalis lanata
NADPH inhibitory at concentrations above 1 mM Digitalis lanata
Zn2+
-
Digitalis lanata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0185 0.02 5alpha-pregnan-3,20-dione pH 8.0, 25°C Digitalis lanata
0.05 0.12 NADPH pH 8.0, 25°C Digitalis lanata

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol major part Digitalis lanata 5829
-
endoplasmic reticulum minor part Digitalis lanata 5783
-
microsome
-
Digitalis lanata
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-pregnan-3,20-dione + NADPH Digitalis lanata
-
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Digitalis lanata
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Digitalis lanata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-pregnan-3,20-dione + NAD(P)H
-
Digitalis lanata 3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+ i.e. 5alpha-pregnan-3beta-ol-20-one r
5alpha-pregnan-3,20-dione + NADPH
-
Digitalis lanata 3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
r

Synonyms

Synonyms Comment Organism
3beta-hydroxysteroid oxidoreductase
-
Digitalis lanata
More 3beta-hydroxysteroid oxidoreductase is exclusively associated with the 5alpha-progesterone reductase in microsomes Digitalis lanata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Digitalis lanata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 35 half-maximal activity at 5°C and 35°C Digitalis lanata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Digitalis lanata

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Digitalis lanata

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Digitalis lanata
NADH
-
Digitalis lanata
NADP+
-
Digitalis lanata
NADPH required Digitalis lanata