BRENDA - Enzyme Database
show all sequences of 1.1.1.277

Cardenolide formation and activity of pregnane-modifying enzymes in cell suspension cultures, shoot cultures and leaves of Digitalis lanata

Stuhlemmer, U.; Kreis, W.; Plant Physiol. Biochem. 34, 85-91 (1996)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Mg2+
-
Digitalis lanata
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Digitalis lanata
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3beta-hydroxy-5beta-pregnane-20-one + NADP+
Digitalis lanata
biosynthesis of cardenolides
5beta-pregnane-3,20-dione + NADPH
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Digitalis lanata
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Digitalis lanata
-
leaf
-
Digitalis lanata
-
shoot
light- and dark-grown
Digitalis lanata
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0003
-
cell suspension culture
Digitalis lanata
0.00084
-
leaf
Digitalis lanata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3beta-hydroxy-5beta-pregnane-20-one + NADP+
stereospecific
389562
Digitalis lanata
5beta-pregnane-3,20-dione + NADPH
-
389562
Digitalis lanata
?
3beta-hydroxy-5beta-pregnane-20-one + NADP+
biosynthesis of cardenolides
389562
Digitalis lanata
5beta-pregnane-3,20-dione + NADPH
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Digitalis lanata
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Digitalis lanata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Mg2+
-
Digitalis lanata
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Digitalis lanata
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3beta-hydroxy-5beta-pregnane-20-one + NADP+
Digitalis lanata
biosynthesis of cardenolides
5beta-pregnane-3,20-dione + NADPH
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Digitalis lanata
-
leaf
-
Digitalis lanata
-
shoot
light- and dark-grown
Digitalis lanata
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0003
-
cell suspension culture
Digitalis lanata
0.00084
-
leaf
Digitalis lanata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3beta-hydroxy-5beta-pregnane-20-one + NADP+
stereospecific
389562
Digitalis lanata
5beta-pregnane-3,20-dione + NADPH
-
389562
Digitalis lanata
?
3beta-hydroxy-5beta-pregnane-20-one + NADP+
biosynthesis of cardenolides
389562
Digitalis lanata
5beta-pregnane-3,20-dione + NADPH
-
-
-
?
Other publictions for EC 1.1.1.277
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389563
Lindemann
-
Biosynthesis of pregnane deriv ...
Digitalis lanata
Phytochemistry
46
507-513
1997
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
2
1
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
2
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389562
Stuhlemmer
-
Cardenolide formation and acti ...
Digitalis lanata
Plant Physiol. Biochem.
34
85-91
1996
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
3
2
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
3
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389560
Seitz
-
Enzymes in cardenolide-accumul ...
Digitalis purpurea
Plant Cell Tissue Organ Cult.
38
337-344
1994
-
-
-
-
-
-
-
3
1
-
-
1
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
1
-
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
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