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Literature summary for 1.1.1.274 extracted from

  • Chen, Y.; Ji, W.; Zhang, H.; Zhang, X.; Yu, Y.
    Cloning, expression and characterization of a putative 2,5-diketo-D-gluconic acid reductase in Comamonas testosteroni (2015), Chem. Biol. Interact., 234, 229-235 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CTATCC11996_22452, genetic organization, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Comamonas testosteroni

Protein Variants

Protein Variants Comment Organism
additional information construction of enzyme gene knockout mutant M-AKR, that shows decreased degradation activity with testosterone, estradiol, oestrone, and methyltestosterone compared to the wild-type enzyme. Compared to the wild-type, the mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-D-gulonate + NADP+ Comamonas testosteroni
-
2,5-didehydro-D-gluconate + NADPH + H+
-
?
2-dehydro-D-gulonate + NADP+ Comamonas testosteroni ATCC 11996
-
2,5-didehydro-D-gluconate + NADPH + H+
-
?
additional information Comamonas testosteroni the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview ?
-
?
additional information Comamonas testosteroni ATCC 11996 the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-
Comamonas testosteroni ATCC 11996
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-D-gulonate + NADP+
-
Comamonas testosteroni 2,5-didehydro-D-gluconate + NADPH + H+
-
?
2-dehydro-D-gulonate + NADP+
-
Comamonas testosteroni ATCC 11996 2,5-didehydro-D-gluconate + NADPH + H+
-
?
additional information the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview Comamonas testosteroni ?
-
?
additional information the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview Comamonas testosteroni ATCC 11996 ?
-
?

Subunits

Subunits Comment Organism
? x * 39400, about, sequence calculation, x * 41600, recombinant His-tagged enzyme, SDS-PAGE Comamonas testosteroni

Synonyms

Synonyms Comment Organism
2,5-diketo-D-gluconic acid reductase
-
Comamonas testosteroni
2,5DKGR
-
Comamonas testosteroni
CTATCC11996_22452
-
Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Comamonas testosteroni

Expression

Organism Comment Expression
Comamonas testosteroni the enzyme expression is significantly induced by estrone, estradiol, and methyltestosterone, and slightly by progesterone, but not by testosterone up

General Information

General Information Comment Organism
evolution the enzyme belongs to the AKR superfamily, monomeric (alpha/beta) 8-barrel proteins which bind NAD(P)(H) to metabolize an array of substrates Comamonas testosteroni
malfunction compared to the wild-type, the knockout mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone. Cell growth on ethanol, oestrone, estradiol, testosterone or methyltestosterone is reduced in the mutant strain compared to the wild-type Comamonas testosteroni
additional information three consensus sequences of the AKR superfamily are found as GxxxxDxAxxY, LxxxGxxxPxxGxG and LxxxxxxxxxDxxxxH. GxxxxDxAxxY is the active site, LxxxGxxxPxxGxG is the cofactor-binding site for NAD(P)(H), and LxxxxxxxxxDxxxxH is required for supporting the 3D structure Comamonas testosteroni