Literature summary for 1.1.1.274 extracted from

Kaswurm, V.; van Hecke, W.; Kulbe, K.; Ludwig, R.
Engineering of a bi-enzymatic reaction for efficient production of the ascorbic acid precursor 2-keto-L-gulonic acid (2013), Biochem. Eng. J., 79, 104-111.

No PubMed abstract available

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Application

Application	Comment	Organism
synthesis	enzymatic production of the vitamin C precursor 2-keto-L-gulonate (2-KLG) from 2,5-diketo-D-gluconate (2,5-DKG) by coupling 2,5-diketo-D-gluconic acid reductase via its coenzyme to glucose dehydrogenase. The bienzymatic process shows complicated inhibition patterns caused by reaction products, NADP+ and NADPH. The key parameters for a fast and efficient conversion are the NADP(H) concentration, the volumetric activity of 2,5-DKG reductase, the ratio of synthetic enzyme activity to regenerate enzyme activity and the glucono-1,5-lactone concentration. By modeling the space-time yield of the process is nearly doubled and the coenzyme concentration reduced threefold	Corynebacterium glutamicum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Corynebacterium glutamicum

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	H9CWC0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,5-didehydro-D-gluconate + NADPH + H+	-	Corynebacterium glutamicum	2-dehydro-D-gluconate + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
Dkr	-	Corynebacterium glutamicum

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Release 2023.1 (January 2023)