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Literature summary for extracted from

  • Kaswurm, V.; van Hecke, W.; Kulbe, K.; Ludwig, R.
    Engineering of a bi-enzymatic reaction for efficient production of the ascorbic acid precursor 2-keto-L-gulonic acid (2013), Biochem. Eng. J., 79, 104-111.
No PubMed abstract available


Application Comment Organism
synthesis enzymatic production of the vitamin C precursor 2-keto-L-gulonate (2-KLG) from 2,5-diketo-D-gluconate (2,5-DKG) by coupling 2,5-diketo-D-gluconic acid reductase via its coenzyme to glucose dehydrogenase. The bienzymatic process shows complicated inhibition patterns caused by reaction products, NADP+ and NADPH. The key parameters for a fast and efficient conversion are the NADP(H) concentration, the volumetric activity of 2,5-DKG reductase, the ratio of synthetic enzyme activity to regenerate enzyme activity and the glucono-1,5-lactone concentration. By modeling the space-time yield of the process is nearly doubled and the coenzyme concentration reduced threefold Corynebacterium glutamicum


Cloned (Comment) Organism
expression in Escherichia coli Corynebacterium glutamicum


Organism UniProt Comment Textmining
Corynebacterium glutamicum H9CWC0

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-didehydro-D-gluconate + NADPH + H+
Corynebacterium glutamicum 2-dehydro-D-gluconate + NADP+


Synonyms Comment Organism
Corynebacterium glutamicum