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Literature summary for 1.1.1.274 extracted from

  • Banta, S.; Swanson, B.A.; Wu, S.; Jarnagin, A.; Anderson, S.
    Optimizing an artificial metabolic pathway: engineering the cofactor specificity of Corynebacterium 2,5-diketo-D-gluconic acid reductase for use in vitamin C biosynthesis (2002), Biochemistry, 41, 6226-6236.
    View publication on PubMed
Search for more literature for 1.1.1.274

Protein Variants

Protein Variants Comment Organism
F22Y/A272G isoenzyme A, reduced kcat for NADH Corynebacterium sp.
F22Y/K232G/R235G/R238E/A272G isoenzyme A, increase in kcat for NADH Corynebacterium sp.
F22Y/K232G/R235G/R238H/A272G isoenzyme A, increase in kcat for NADH Corynebacterium sp.
F22Y/K232G/R235T/R238E/A272G isoenzyme A, 24fold increase in kcat for NADH Corynebacterium sp.
F22Y/K232G/R238H/A272G isoenzyme A, increase in kcat forNADH Corynebacterium sp.
F22Y/S232T/R235S/R238H/A272G isoenzyme A, increase in kcat for NADH Corynebacterium sp.
K232G/R238H isoenzyme A, increase in kcat for NADH Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 NADPH F22Y/A272G isoenzyme A mutant Corynebacterium sp.
0.055
-
 NADPH F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant Corynebacterium sp.
0.058
-
 NADPH F22Y/K232G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
0.071
-
 NADPH F22Y/K232G/R235T/R238H/A272G isoenzyme A mutant Corynebacterium sp.
0.13
-
 NADPH K232G/R238H isoenzyme A mutant Corynebacterium sp.
0.15
-
 NADPH F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
0.39
-
 2,5-didehydro-D-gluconate F22Y/K232G/R235T/R238H/A272G isoenzyme A mutant Corynebacterium sp.
0.66
-
 NADH K232G/R238H isoenzyme A mutant Corynebacterium sp.
1.2
-
 NADH F22Y/K232G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
1.4
-
 NADH isoenzyme A, wild-type Corynebacterium sp.
2.4
-
 NADH F22Y/A272G isoenzyme A mutant Corynebacterium sp.
2.7
-
 NADH F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
3.4
-
 NADH F22Y/K232G/R235G/R238E/A272G isoenzyme A mutant Corynebacterium sp.
3.9
-
 NADH F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant Corynebacterium sp.
5.2
-
 NADH F22Y/K232G/R235T/R238E/A272G isoenzyme A mutant Corynebacterium sp.
7.2
-
 2,5-didehydro-D-gluconate F22Y/A272G isoenzyme A mutant Corynebacterium sp.
8.7
-
 2,5-didehydro-D-gluconate F22Y/K232G/R235T/R238H/A272G isoenzyme A mutant Corynebacterium sp.
9.1
-
 2,5-didehydro-D-gluconate F22Y/K232G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
13
-
 2,5-didehydro-D-gluconate K232G/R238H isoenzyme A mutant Corynebacterium sp.
13
-
 2,5-didehydro-D-gluconate F22Y/K232G/R235G/R238E/A272G isoenzyme A mutant Corynebacterium sp.
27
-
 2,5-didehydro-D-gluconate F22Y/S232T/R235S/R238H/A272G isoenzyme A mutant Corynebacterium sp.
30
-
 2,5-didehydro-D-gluconate F22Y/A272G isoenzyme A mutant Corynebacterium sp.
32
-
 2,5-didehydro-D-gluconate F22Y/K232G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
32
-
 2,5-didehydro-D-gluconate F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
43
-
 2,5-didehydro-D-gluconate F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
110
-
 2,5-didehydro-D-gluconate K232G/R238H isoenzyme A mutant Corynebacterium sp.
130
-
 2,5-didehydro-D-gluconate isoenzyme A, wild-type Corynebacterium sp.
150
-
 2,5-didehydro-D-gluconate F22Y/K232G/R235T/R238E/A272G isoenzyme A mutant Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-didehydro-D-gluconate + NADH
-
 Corynebacterium sp. 2-keto-L-gulonate + NAD+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.23
-
 NADH F22Y/A272G isoenzyme A mutant Corynebacterium sp.
2.17
-
 NADPH F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant Corynebacterium sp.
2.83
-
 NADH isoenzyme A, wild-type Corynebacterium sp.
3.33
-
 NADPH K232G/R238H isoenzyme A mutant Corynebacterium sp.
7
-
 NADPH F22Y/K232G/R235T/R238H/A272G isoenzyme A mutant Corynebacterium sp.
7.33
-
 NADPH F22Y/K232G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
8.5
-
 NADH F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant Corynebacterium sp.
10
-
 NADH F22Y/K232G/R235G/R238E/A272G isoenzyme A mutant Corynebacterium sp.
16.5
-
 NADH F22Y/K232G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
18.3
-
 NADPH F22Y/A272G isoenzyme A mutant Corynebacterium sp.
18.3
-
 NADPH F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
18.3
-
 NADH F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant Corynebacterium sp.
20
-
 NADH K232G/R238H isoenzyme A mutant Corynebacterium sp.
68.3
-
 NADH F22Y/K232G/R235T/R238E/A272G isoenzyme A mutant Corynebacterium sp.