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Literature summary for 1.1.1.273 extracted from

  • Pfitzner, A.; Krausch, B.; Stoeckigt, J.
    Characteristics of vellosimine reductase, a specific enzyme involved in the biosynthesis of the Rauwolfia alkaloid sarpagine (1984), Tetrahedron, 40, 1691-1699.
No PubMed abstract available
Search for more literature for 1.1.1.273

General Stability

General Stability Organism
highly instable enzyme, in crude extracts, half-life of activity is 25 h at 4°C, 0.25% activity remains after 7 d, activity depends completely on the presence of 2-mercaptoethanol Rauvolfia serpentina

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 71% inhibition Rauvolfia serpentina
EDTA 1 mM, 77% inhibition Rauvolfia serpentina
NADP+ noncompetitive inhibition Rauvolfia serpentina
neocuproin 1 mM, 65% inhibition Rauvolfia serpentina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0167
-
 vellosimine
-
 Rauvolfia serpentina
0.02
-
 NADPH
-
 Rauvolfia serpentina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
-
 Rauvolfia serpentina

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-
no activity in Alstonia scholaris
-
-
-
no activity in Carissa macrocarpa
-
-
-
no activity in Catharanthus roseus
-
-
-
no activity in Coleus blumei
-
-
-
no activity in Malus sylvestris
-
-
-
no activity in Nicotiana sylvestris
-
-
-
no activity in Picralima nitida
-
-
-
no activity in Rhazya stricta
-
-
-
no activity in Stemmadenia tomentosa
-
-
-
no activity in Tabernanthe iboga
-
-
-
no activity in Voacanga africana
-
-
-
Rauvolfia serpentina
-
-
-
Rauvolfia verticillata
-
-
-
Rauvolfia vomitoria
-
-
-
Vinca herbacea
-
-
-
Vinca minor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rauvolfia serpentina

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Rauvolfia serpentina
-
cell suspension culture
-
 Rauvolfia verticillata
-
cell suspension culture
-
 Rauvolfia vomitoria
-
cell suspension culture
-
 Catharanthus roseus
-
cell suspension culture
-
 Vinca minor
-
cell suspension culture
-
 Vinca herbacea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00018
-
 vellosimine reduction in cell suspension culture Vinca herbacea
0.0006
-
 vellosimine reduction in cell suspension culture Vinca minor
0.0012
-
-
 Rauvolfia serpentina
0.013
-
 vellosimine reduction in cell suspension culture Rauvolfia vomitoria
0.0156
-
 vellosimine reduction in cell suspension culture Catharanthus roseus
0.0192
-
 vellosimine reduction in cell suspension culture Rauvolfia verticillata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
16-epi-vellosimine + NADPH
-
 Rauvolfia serpentina ?
-
 ?
deoxyajmalal B + NADPH
-
 Rauvolfia serpentina ?
-
 ?
gardneral + NADPH
-
 Rauvolfia serpentina ?
-
 ?
geissoschizal + NADPH
-
 Rauvolfia serpentina ?
-
 ?
N-methylgardneral + NADPH
-
 Rauvolfia serpentina ?
-
 ?
vellosimine + NADPH
-
 Rauvolfia serpentina 10-deoxysarpagine + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Rauvolfia serpentina

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 50% of maximal activity at pH 6.8 and pH 7.6 respectively Rauvolfia serpentina

Cofactor

Cofactor Comment Organism Structure
NADPH absolutely specific for NADPH Rauvolfia serpentina