Application | Comment | Organism |
---|---|---|
synthesis | used of mutated 3-ketosteroid reductase (Erg27) to obtain radioactive and non-radioactive intermediates of sterol biosynthesis hardly or not available commercially. The strategies are either incubation of cell homogenates of an Erg27-deletant strain with radioactive lanosterol, or incubation of growing cells of a strain expressing point-mutated 3-ketosteroid reductase with radioactive acetate. Chemical reduction of both radioactive and non-radioactive 3-keto sterones gives the physiological 3-beta-OH sterols, as well as the non-physiological 3-alpha-OH isomers | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
K206A | strain can be used to obtain radioactive and non-radioactive intermediates of sterol biosynthesis hardly or not available commercially | Saccharomyces cerevisiae |
additional information | labelled C-4 methylsterones can be obtained from Erg27-deletant strains by incubating a cell-free homogenate with radioactive lanosterol | Saccharomyces cerevisiae |
R40A | strain can be used as a source of 4-methylzymosterone | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12452 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Erg27 | - |
Saccharomyces cerevisiae |