Cloned (Comment) | Organism |
---|---|
isoform type III of 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase with almost exclusive 3-ketosteroid reductase activity, cDNA expression | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trilostane | IC50: 0.00023 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
NADPH | substrate: 5alpha-dihydrotestosterone, DHT | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | catalyzes the conversion of 3-keto-saturated steroids such as 5alpha-dihydrotestosterone, DHT, and 5alpha-androstane-3,17-dione, A-dione, into less active steroids | ? | - |
? | |
additional information | Rattus norvegicus | step of biosynthesis of hormonal steroids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male rat | - |
Rattus norvegicus | - |
isoform type III of 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase with almost exclusive 3-ketosteroid reductase activity | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydroxy-5alpha-androstan-3-one + NADPH | 5alpha-dihydrotestosterone | Rattus norvegicus | 5alpha-androstane-3beta,17beta-diol + NADP+ | androstanediol | ir | |
17beta-hydroxy-5alpha-androstan-3-one + NADPH | 5alpha-dihydrotestosterone | Rattus norvegicus | 5alpha-androstane-3beta,17beta-diol + NADP+ | corresponding 3beta-hydroxysteroid | ir | |
17beta-hydroxy-5alpha-androstan-3-one + NADPH | DHT | Rattus norvegicus | 5alpha-androstane-3beta,17beta-diol + NADP+ | androstanediol | ir | |
17beta-hydroxy-5alpha-androstan-3-one + NADPH | DHT | Rattus norvegicus | 5alpha-androstane-3beta,17beta-diol + NADP+ | corresponding 3beta-hydroxysteroid | ir | |
17beta-hydroxy-5alpha-androstan-3-one + NADPH | dihydrotestosterone | Rattus norvegicus | 5alpha-androstane-3beta,17beta-diol + NADP+ | androstanediol | ir | |
17beta-hydroxy-5alpha-androstan-3-one + NADPH | dihydrotestosterone | Rattus norvegicus | 5alpha-androstane-3beta,17beta-diol + NADP+ | corresponding 3beta-hydroxysteroid | ir | |
3-keto-5alpha-androstane steroids + NADPH | - |
Rattus norvegicus | 3beta-hydroxy-5alpha-androstane steroids + NADP+ | - |
ir | |
5alpha-androstane-3,17-dione + NADPH | A-dione | Rattus norvegicus | epi-androsterone + NADP+ | - |
ir | |
additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
additional information | catalyzes the conversion of 3-keto-saturated steroids such as 5alpha-dihydrotestosterone, DHT, and 5alpha-androstane-3,17-dione, A-dione, into less active steroids | Rattus norvegicus | ? | - |
? | |
additional information | step of biosynthesis of hormonal steroids | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | NADPH preferred cofactor for 3-ketoreductase activity expressed type III isoform | Rattus norvegicus | |
NADPH | specific for NADPH, NADH is essentially inactive, less than 10% | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00023 | - |
IC50: 0.00023 mM | Rattus norvegicus | trilostane |