BRENDA - Enzyme Database show
show all sequences of 1.1.1.270

A DNA-binding of mouse kidney 3-ketosteroid reductase: comparison with androgen receptors

Wieland, S.J.; Fox, T.O.; Steroids 37, 527-538 (1981)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Mus musculus
5737
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
enzyme might form complexes with other proteins, including possibly DNA-binding proteins, under low ionic strength conditions
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
wild-type
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Mus musculus
-
hypothalamus
-
Mus musculus
-
kidney
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17beta-hydroxy-5alpha-androstan-3-one + NADPH
5alpha-dihydrotestosterone
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
androstanediol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
5alpha-dihydrotestosterone
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
5alpha-androstane-3alpha,17beta-diol or 5alpha-androstane-3beta,17beta-diol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
DHT
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
androstanediol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
DHT
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
5alpha-androstane-3alpha,17beta-diol or 5alpha-androstane-3beta,17beta-diol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
dihydrotestosterone
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
androstanediol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
dihydrotestosterone
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
5alpha-androstane-3alpha,17beta-diol or 5alpha-androstane-3beta,17beta-diol
286475
Mus musculus
?
additional information
enzyme might form complexes with other proteins, including possibly DNA-binding proteins, under low ionic strength conditions
286475
Mus musculus
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Mus musculus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
-
Mus musculus
25
-
2 h at 25C: 84% of activity remained, 8 h at 25C: 74-76% of activity remained
Mus musculus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Mus musculus
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
enzyme might form complexes with other proteins, including possibly DNA-binding proteins, under low ionic strength conditions
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Mus musculus
-
hypothalamus
-
Mus musculus
-
kidney
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17beta-hydroxy-5alpha-androstan-3-one + NADPH
5alpha-dihydrotestosterone
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
androstanediol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
5alpha-dihydrotestosterone
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
5alpha-androstane-3alpha,17beta-diol or 5alpha-androstane-3beta,17beta-diol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
DHT
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
androstanediol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
DHT
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
5alpha-androstane-3alpha,17beta-diol or 5alpha-androstane-3beta,17beta-diol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
dihydrotestosterone
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
androstanediol
286475
Mus musculus
?
17beta-hydroxy-5alpha-androstan-3-one + NADPH
dihydrotestosterone
286475
Mus musculus
5alpha-androstane-3beta,17beta-diol + NADP+
5alpha-androstane-3alpha,17beta-diol or 5alpha-androstane-3beta,17beta-diol
286475
Mus musculus
?
additional information
enzyme might form complexes with other proteins, including possibly DNA-binding proteins, under low ionic strength conditions
286475
Mus musculus
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Mus musculus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
-
Mus musculus
25
-
2 h at 25C: 84% of activity remained, 8 h at 25C: 74-76% of activity remained
Mus musculus
Other publictions for EC 1.1.1.270
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740936
Ferrante
4-Methylzymosterone and other ...
Saccharomyces cerevisiae
Lipids
51
1103-1113
2016
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741250
Chen
Metabolism of androstenone, 17 ...
Sus scrofa
PLoS ONE
10
e113194
2015
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726098
Debieu
Role of sterol 3-ketoreductase ...
Botrytis cinerea
Pest Manag. Sci.
69
642-651
2013
-
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1
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5
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1
1
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5
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3
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1
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1
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2
2
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740097
Layer
Characterization of a mutation ...
Mus musculus
Biochim. Biophys. Acta
1831
361-369
2013
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4
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1
1
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724124
Endo
Characterization of rabbit ald ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
527
23-30
2012
-
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1
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4
-
14
20
1
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1
1
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3
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1
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6
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27
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1
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21
1
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2
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14
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1
2
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4
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14
14
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20
1
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1
1
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1
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6
-
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27
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1
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21
1
-
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3
3
-
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-
702462
Taramino
Interactions of oxidosqualene ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1801
156-162
2010
-
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1
1
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711300
Taramino
Divergent interactions involvi ...
Homo sapiens, Mus musculus
Biochim. Biophys. Acta
1801
1232-1237
2010
-
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2
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7
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1
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1
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1
1
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725813
Thomas
The functions of key residues ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
120
192-199
2010
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1
1
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6
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1
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2
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1
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6
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7
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1
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4
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1
2
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6
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1
4
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1
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6
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7
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1
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1
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3
3
-
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673822
Tam
Activities of 3beta-HSD and ar ...
Taeniopygia guttata
Gen. Comp. Endocrinol.
150
26-33
2006
-
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5
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3
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675595
Steckelbroeck
Tibolone metabolism in human l ...
Homo sapiens
J. Pharmacol. Exp. Ther.
316
1300-1309
2006
-
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1
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2
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1
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3
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1
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1
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677144
Jin
Molecular docking simulations ...
Homo sapiens
Steroids
71
380-391
2006
-
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2
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2
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656200
Steckelbroeck
Human cytosolic 3alpha-hydroxy ...
Homo sapiens
J. Biol. Chem.
279
10784-10795
2004
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1
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3
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2
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3
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3
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655348
Rizner
Human type 3 3alpha-hydroxyste ...
Homo sapiens
Endocrinology
144
2922-2932
2003
-
-
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1
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4
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3
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656795
Marijanovic
Closing the gap: Identificatio ...
Homo sapiens, Mus musculus
Mol. Endocrinol.
17
1715-1725
2003
-
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2
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1
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2
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2
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5
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5
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5
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4
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723672
Mo
Protein-protein interactions a ...
Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
99
39-44
2002
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286477
Huang
Gene structure, chromosomal lo ...
Homo sapiens
Biochim. Biophys. Acta
1520
124-130
2001
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1
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3
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286474
Pascal
Plant sterol biosynthesis: ide ...
Zea mays
Arch. Biochem. Biophys.
312
260-271
1994
1
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2
11
2
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27
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11
2
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2
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27
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286476
Vanden Bossche
Effects of itraconazole on cyt ...
Cryptococcus neoformans
Antimicrob. Agents Chemother.
37
2101-2105
1993
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1
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286478
De Launoit
Expression of liver-specific m ...
Rattus norvegicus
J. Biol. Chem.
267
4513-4517
1992
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1
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1
1
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11
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1
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1
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11
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1
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1
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286473
Appelkvist
Presence of individual enzymes ...
Rattus norvegicus
Arch. Biochem. Biophys.
282
318-325
1990
1
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4
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2
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2
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1
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1
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286472
Billheimer
Solubilization and partial pur ...
Rattus norvegicus
Arch. Biochem. Biophys.
211
430-438
1981
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2
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7
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1
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286475
Wieland
A DNA-binding of mouse kidney ...
Mus musculus
Steroids
37
527-538
1981
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