Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose-1,6-bisphosphate | enzyme binding structure, overview. The allosteric enzyme requires fructose-1,6-bisphosphate (FBP), an intermediate in the glycolysis pathway, for catalytic activities. FBP may play a role in stabilizing and maintaining the tetrameric structure | Enterococcus mundtii | |
D-fructose-1,6-bisphosphate | the allosteric enzyme requires fructose-1,6-bisphosphate (FBP), an intermediate in the glycolysis pathway, for catalytic activities. FBP may play a role in stabilizing and maintaining the tetrameric structure | Enterococcus mundtii |
Cloned (Comment) | Organism |
---|---|
gene ldh-1, DNA and amino acid sequence determinaation and analysis, sequence comparisons, recombinant expression in Escherichia coli | Enterococcus mundtii |
gene ldh-2, DNA and amino acid sequence determinaation and analysis, sequence comparisons, recombinant expression in Escherichia coli | Enterococcus mundtii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type enzyme, in apoform and in complex with fructose-1,6-bisphosphate and NADH, X-ray diffraction structure determination and analysis at 2.38 A and 2.30 A resolution, respectively, modelling | Enterococcus mundtii |
Protein Variants | Comment | Organism |
---|---|---|
D241N | site-directed mutagenesis, the mutant hsows higher activity at pH above 5.5 compared to the wild-type enzyme | Enterococcus mundtii |
E60Q | site-directed mutagenesis, the mutant has a similar pH prodile as the wild-type | Enterococcus mundtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | alternative allosteric regulation mechanism of an acidophilic L-lactate dehydrogenase, kinetic analysis of the recombinant enzyme, overview | Enterococcus mundtii | |
0.11 | - |
NADH | pH 7.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
0.18 | - |
NADH | pH 5.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
0.2 | - |
NADH | pH 5.5, temperature not specified in the publication, mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
0.26 | - |
NADH | pH 7.5, temperature not specified in the publication, mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
0.41 | - |
NADH | pH 5.5, temperature not specified in the publication, wild-type LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
0.9 | - |
NADH | pH 5.5, temperature not specified in the publication, mutant D241N LDH-2, without fructose-1,6-bisphosphate | Enterococcus mundtii | |
0.93 | - |
pyruvate | pH 5.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
0.93 | - |
NADH | pH 7.5, temperature not specified in the publication, wild-type LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
2.2 | - |
pyruvate | pH 5.5, temperature not specified in the publication, wild-type LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
2.3 | - |
pyruvate | pH 5.5, temperature not specified in the publication, mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
2.7 | - |
pyruvate | pH 7.5, temperature not specified in the publication, wild-type LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
3 | - |
pyruvate | pH 7.5, temperature not specified in the publication, mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
3.8 | - |
pyruvate | pH 7.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
15 | - |
pyruvate | pH 5.5, temperature not specified in the publication, mutant D241N LDH-2, without fructose-1,6-bisphosphate | Enterococcus mundtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | Enterococcus mundtii | - |
pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | Enterococcus mundtii 15-1A | - |
pyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus mundtii | - |
isolated from Brassica rapa L. subsp. nipposinica (L.H. Bailey) Hanelt var. linearifolia | - |
Enterococcus mundtii | A0A1A6GB48 | LDH-1; isolated from Brassica rapa L. subsp. nipposinica (L.H. Bailey) Hanelt var. linearifolia | - |
Enterococcus mundtii 15-1A | - |
isolated from Brassica rapa L. subsp. nipposinica (L.H. Bailey) Hanelt var. linearifolia | - |
Enterococcus mundtii 15-1A | A0A1A6GB48 | LDH-1; isolated from Brassica rapa L. subsp. nipposinica (L.H. Bailey) Hanelt var. linearifolia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Enterococcus mundtii | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | - |
Enterococcus mundtii 15-1A | pyruvate + NADH + H+ | - |
r | |
additional information | substrate binding structure, overview. The side chain of the Arg171 residue of LDH-2 seems to be important for the binding of pyruvate, pointing toward the pyruvate-binding site | Enterococcus mundtii | ? | - |
? | |
additional information | substrate binding structure, overview. The side chain of the Arg171 residue of LDH-2 seems to be important for the binding of pyruvate, pointing toward the pyruvate-binding site | Enterococcus mundtii 15-1A | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | each monomer has one active site, and the tetramer has two allosteric sites, each of which is situated at the Y-axis interface between two monomers | Enterococcus mundtii |
More | secondary, tertiary, and quaternary structure analysis, and structure comparisons, overview.The subunit structure of L-LDH can be divided into N-terminal NADH-binding domain and C-terminal catalytic domain | Enterococcus mundtii |
Synonyms | Comment | Organism |
---|---|---|
L-LDH | - |
Enterococcus mundtii |
LDH-1 | - |
Enterococcus mundtii |
LDH-2 | - |
Enterococcus mundtii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1300 | - |
pyruvate | pH 7.5, temperature not specified in the publication, wild-type LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
1500 | - |
pyruvate | pH 7.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
1600 | - |
pyruvate | pH 5.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
1800 | - |
pyruvate | pH 5.5, temperature not specified in the publication, wild-type and mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
1800 | - |
pyruvate | pH 7.5, temperature not specified in the publication, mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
detailed kinetic analysis of enzyme mutant D241N, done at pH 5.5 and pH 7.5 | Enterococcus mundtii |
5.5 | - |
- |
Enterococcus mundtii |
5.5 | - |
maximal activity at pH 5.5 which gradually decreases with the increase of pH | Enterococcus mundtii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | activity range, inactive at pH 8.0, profile overview | Enterococcus mundtii |
4 | 7.5 | activity range, inactive at pH 8.0, profile overview. High level of activity between pH 4.0 and pH 7.5 | Enterococcus mundtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Enterococcus mundtii | |
NADH | - |
Enterococcus mundtii | |
NADH | enzyme binding structure, overview | Enterococcus mundtii |
General Information | Comment | Organism |
---|---|---|
evolution | sequence identity between the Enterococcus mundtii LDH-1 and LDH-2 is 44.9% | Enterococcus mundtii |
physiological function | alternative allosteric regulation mechanism of an acidophilic L-lactate dehydrogenase, overview. LDH-1 mainly plays a role in L-lactate production in Enterococcus mundtii, while LDH-2 plays another, different role | Enterococcus mundtii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
pyruvate | pH 7.5, temperature not specified in the publication, mutant D241N LDH-2, without fructose-1,6-bisphosphate | Enterococcus mundtii | |
59 | - |
pyruvate | pH 5.5, temperature not specified in the publication, mutant D241N LDH-2, without fructose-1,6-bisphosphate | Enterococcus mundtii | |
200 | - |
NADH | pH 7.5, temperature not specified in the publication, mutant D241N LDH-2, without fructose-1,6-bisphosphate | Enterococcus mundtii | |
390 | - |
pyruvate | pH 7.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
480 | - |
pyruvate | pH 7.5, temperature not specified in the publication, wild-type LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
610 | - |
pyruvate | pH 7.5, temperature not specified in the publication, mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
790 | - |
pyruvate | pH 5.5, temperature not specified in the publication, mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
810 | - |
pyruvate | pH 5.5, temperature not specified in the publication, wild-type LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
1200 | - |
NADH | pH 5.5, temperature not specified in the publication, mutant D241N LDH-2, without fructose-1,6-bisphosphate | Enterococcus mundtii | |
1400 | - |
NADH | pH 7.5, temperature not specified in the publication, wild-type LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
1700 | - |
pyruvate | pH 5.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
4400 | - |
NADH | pH 5.5, temperature not specified in the publication, wild-type LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
6800 | - |
NADH | pH 7.5, temperature not specified in the publication, mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
9000 | - |
NADH | pH 5.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
9000 | - |
NADH | pH 5.5, temperature not specified in the publication, mutant D241N LDH-2, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii | |
13000 | - |
NADH | pH 7.5, temperature not specified in the publication, LDH-1, with 3 mM fructose-1,6-bisphosphate | Enterococcus mundtii |