Cloned (Comment) | Organism |
---|---|
gene AA02769 or lctD, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli DH5alpha, expression as His6-tagged protein in Escherichia coli BL21(DE3) | Aggregatibacter actinomycetemcomitans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an insertion mutant of gene AA02769, inactivation of AA02769 eliminates the ability of the organism to grow on L-lactate, but not on D-glucose | Aggregatibacter actinomycetemcomitans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the Aggregatibacter actinomycetemcomitans L-lactate dehydrogenase, unlike homologous enzymes, is not feedback inhibited by pyruvate, pyruvate is a poor inhibitor of L-lactate dehydrogenase activity | Aggregatibacter actinomycetemcomitans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.152 | - |
L-lactate | pH not specified in the publication, temperature not specified in the publication | Aggregatibacter actinomycetemcomitans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, recombinant His6-tagged LctD, SDS-PAGE | Aggregatibacter actinomycetemcomitans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | Aggregatibacter actinomycetemcomitans | no activity with D-lactate | pyruvate + NADH + H+ | - |
r | |
L-lactate + NAD+ | Aggregatibacter actinomycetemcomitans HK1651 and VT1169 | no activity with D-lactate | pyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aggregatibacter actinomycetemcomitans | - |
a Gram-negative opportunistic pathogen found exclusively in the mammalian oral cavity in the space between the gums and the teeth known as the gingival crevice, gene AA02769 | - |
Aggregatibacter actinomycetemcomitans HK1651 and VT1169 | - |
a Gram-negative opportunistic pathogen found exclusively in the mammalian oral cavity in the space between the gums and the teeth known as the gingival crevice, gene AA02769 | - |
Purification (Comment) | Organism |
---|---|
native enzyme, and recombinant His6-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3) | Aggregatibacter actinomycetemcomitans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | no activity with D-lactate | Aggregatibacter actinomycetemcomitans | pyruvate + NADH + H+ | - |
r | |
L-lactate + NAD+ | no activity with D-lactate, activity of recombinant His6-tagged LctD is determined in the presence of the electron carriers 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide and phenazine methosulfate | Aggregatibacter actinomycetemcomitans | pyruvate + NADH + H+ | - |
r | |
L-lactate + NAD+ | no activity with D-lactate | Aggregatibacter actinomycetemcomitans HK1651 and VT1169 | pyruvate + NADH + H+ | - |
r | |
L-lactate + NAD+ | no activity with D-lactate, activity of recombinant His6-tagged LctD is determined in the presence of the electron carriers 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide and phenazine methosulfate | Aggregatibacter actinomycetemcomitans HK1651 and VT1169 | pyruvate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, recombinant His6-tagged LctD, SDS-PAGE | Aggregatibacter actinomycetemcomitans |
Synonyms | Comment | Organism |
---|---|---|
LctD | - |
Aggregatibacter actinomycetemcomitans |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first step in L-lactate catabolism | Aggregatibacter actinomycetemcomitans |