Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of ldh1 and ldh-2 deletion mutants and of a catalytically inactive double knockout mutant of genes ldh-1 and ldh-2 by gene replacement achieved by double-crossover homologous recombination. The ldh-2 mutant shows hardly any differences in growth and metabolism in comparison to the wild-type. The two mutants lacking ldh-1, on the other hand, produce much less lactate than the wild-type but grew to higher cell densities and have higher final pHs, phenotypes, overview | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | Enterococcus faecalis | the wild-type V583 strain converts glucose almost exclusively to L-lactate under anaerobic conditions | pyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
genes ldh-1 and ldh-2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | - |
Enterococcus faecalis | pyruvate + NADH + H+ | - |
r | |
L-lactate + NAD+ | the wild-type V583 strain converts glucose almost exclusively to L-lactate under anaerobic conditions | Enterococcus faecalis | pyruvate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
LDH-1 | - |
Enterococcus faecalis |
LDH-2 | - |
Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Enterococcus faecalis | |
NADH | - |
Enterococcus faecalis |
General Information | Comment | Organism |
---|---|---|
additional information | the ldh-1 mutants detoxifies excess pyruvate by converting it to acetoin | Enterococcus faecalis |
physiological function | although Ldh-2 contributes to lactate production, Ldh-1 plays the major role in energy metabolism in Enterococcus faecalis | Enterococcus faecalis |