Protein Variants | Comment | Organism |
---|---|---|
additional information | the malate dehydrogenase, EC 1.1.1.37, mutant I12V/R81Q/M85E/G210A/V214I shows a substrate specificity that is switched from malate dehydrogenase to that of lactate dehydrogenase, overview | Escherichia coli |
additional information | the malate dehydrogenase, EC 1.1.1.37, mutant I12V/R81Q/M85E/G210A/V214I shows a substrate specificity that is switched from malate dehydrogenase to that of lactate dehydrogenase, overview | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
pyruvate | pH 6.0, 25°C, recombinant wild-type enzyme | Geobacillus stearothermophilus | |
13.3 | - |
pyruvate | pH 7.5, 25°C, recombinant mutant I12V/R81Q/M85E/G210A/V214I | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | Geobacillus stearothermophilus | - |
pyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Geobacillus stearothermophilus | P00344 | gene ldh | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Geobacillus stearothermophilus | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | mutant I12V/R81Q/M85E/G210A/V214I, residues I12, R81, M85, G210, and V214 determine the enzyme's substrate specificity | Escherichia coli | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | residues I12, R81, M85, G210, and V214 determine the enzyme's substrate specificity | Geobacillus stearothermophilus | pyruvate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
lactate dehydrogenase | - |
Escherichia coli |
lactate dehydrogenase | - |
Geobacillus stearothermophilus |
LDH | - |
Escherichia coli |
LDH | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Geobacillus stearothermophilus |
30 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46.5 | - |
pyruvate | pH 7.5, 25°C, recombinant mutant I12V/R81Q/M85E/G210A/V214I | Escherichia coli | |
250 | - |
pyruvate | pH 6.0, 25°C, recombinant wild-type enzyme | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Geobacillus stearothermophilus |
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NAD+ | - |
Geobacillus stearothermophilus | |
NADH | - |
Escherichia coli | |
NADH | - |
Geobacillus stearothermophilus |