Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Clupea harengus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
pyruvate | pH 7.2, 25°C, isoenzyme LDH-A2B2 | Clupea harengus | |
0.09 | - |
pyruvate | pH 7.6, 25°C, isoenzyme LDH-B4 | Clupea harengus | |
0.22 | - |
pyruvate | pH 7.1, 25°C, isoenzyme LDH-A4 | Clupea harengus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clarias gariepinus | - |
- |
- |
Clupea harengus | - |
- |
- |
Cyprinus carpio | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzyme LDH-A4 from muscle | Clupea harengus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | isoenzyme LDH-A4 | Clupea harengus | - |
spermatozoon | - |
Cyprinus carpio | - |
spermatozoon | - |
Clarias gariepinus | - |
spermatozoon | isoenzyme LDH-B4 and LDH-A2B2 | Clupea harengus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Cyprinus carpio | (S)-lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Clarias gariepinus | (S)-lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Clupea harengus | (S)-lactate + NAD+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min, about 10% loss of activity, isoenzyme A4 | Clupea harengus |
50 | - |
20 min, about 40% loss of activity of isoenzyme A4, about 10% loss of activity of isoenzyme B4, about 25% loss of activity of isoenzyme A2B2 | Clupea harengus |
51 | - |
20 min, about 75% loss of activity, isoenzyme A4 | Clupea harengus |
53 | - |
20 min, complete loss of activity of isoenzyme A4, about 40% loss of activity of isoenzyme A2B2 | Clupea harengus |
55 | - |
20 min, about 90% loss of activity of isoenzyme A2B2, about 15% loss of activity of isoenzyme B4 | Clupea harengus |
66 | - |
20 min, about 35% loss of activity of isoenzyme B4 | Clupea harengus |
68 | - |
20 min, about 68% loss of activity of isoenzyme B4 | Clupea harengus |
71 | - |
20 min, complete loss of activity of isoenzyme B4 | Clupea harengus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
reduction of pyruvate, isoenzyme LDH-A4 | Clupea harengus |
7.25 | - |
reduction of pyruvate, isoenzyme LDH-A2B2 | Clupea harengus |
7.6 | - |
reduction of pyruvate, isoenzyme LDH-B4 | Clupea harengus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 5.5: 40% of maximal activity of isoenzyme LDH-B4 and 60% of maximal activity of isoenzyme LDH-A2B2 and LDH-A4, pH 8.0: about 60% of maximal activity of isoenzyme LDH-A4, LDH-B4 and LDH-A4B4, reduction of pyruvate | Clupea harengus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Cyprinus carpio | |
NADH | - |
Clarias gariepinus | |
NADH | - |
Clupea harengus |