Protein Variants | Comment | Organism |
---|---|---|
S163L | decrease in substrate inhibition, the Km value for pyruvate is increased substantially and the turnover number is 60% that of wild type | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | lack of substrate inhibition | Plasmodium falciparum | |
pyruvate | substrate inhibition in wild type enzyme, lower substrate inhibition in mutant S163L | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-values for mutant enzymes with enlarged loop | Plasmodium falciparum | |
0.018 | - |
NADH | wild-type enzyme | Plasmodium falciparum | |
0.065 | - |
NADH | mutant enzyme S163L | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and enlarged loop mutants ELM1 and ELM2 | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-2-oxopentanoate + NADH | - |
Plasmodium falciparum | 2-hydroxy-4-methylpentanoate + NAD+ | - |
? | |
phenylpyruvate + NADH | - |
Plasmodium falciparum | 2-hydroxy-3-phenylpropanoate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Plasmodium falciparum | (S)-lactate + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover-numbers for mutant enzymes with enlarged loop | Plasmodium falciparum | |
0.33 | - |
4-methyl-2-oxopentanoate | wild-type enzyme | Plasmodium falciparum | |
39.5 | - |
phenylpyruvate | wild-type enzyme | Plasmodium falciparum | |
180 | - |
pyruvate | wild-type enzyme | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine adenine dinucleotide | wild-type and S163 L mutant protein bind 3-acetylpyridine adenine dinucleotide more tightly than they bind NADH | Plasmodium falciparum | |
NADH | coenzyme | Plasmodium falciparum |