Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-diphosphate | L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate | Lactococcus cremoris | |
glucose 1,6-diphosphate | activates | Lactococcus cremoris | |
tagatose 1,6-diphosphate | stimulates | Lactococcus cremoris |
General Stability | Organism |
---|---|
NADH and fructose 1,6-diphosphate partially stabilize the 140000 Da molecular weight form against dissociation in triethanolamine-hydrochloride buffer at pH 8.0 | Lactococcus cremoris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Lactococcus cremoris | |
oxamate | - |
Lactococcus cremoris | |
p-chloromercuribenzoate | - |
Lactococcus cremoris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
NADH | in presence of fructose 1,6-diphosphate | Lactococcus cremoris | |
1.15 | - |
pyruvate | in absence of fructose 1,6-diphosphate | Lactococcus cremoris | |
7.7 | - |
pyruvate | in presence of fructose 1,6-diphosphate | Lactococcus cremoris | |
44 | - |
NADH | in absence of fructose 1,6-diphosphate | Lactococcus cremoris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
ultracentrifugal studies | Lactococcus cremoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus cremoris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus cremoris |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-lactate + NAD+ = pyruvate + NADH + H+ | reduction of pyruvate follows an Theorell-Chance mechanism | Lactococcus cremoris |
Storage Stability | Organism |
---|---|
-20°C, 50 mM sodium phosphate buffer, pH 7.0, stable for several months | Lactococcus cremoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Lactococcus cremoris | (S)-lactate + NAD+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
protection against heat inactivation by 1 mM fructose 1,6-diphosphate, 1 mM ATP, 1 mM glucose 6-phosphate and increased levels of phosphate | Lactococcus cremoris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | coenzyme | Lactococcus cremoris |