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Literature summary for 1.1.1.265 extracted from

  • Guo, P.C.; Bao, Z.Z.; Ma, X.X.; Xia, Q.; Li, W.F.
    Structural insights into the cofactor-assisted substrate recognition of yeast methylglyoxal/isovaleraldehyde reductase Gre2 (2014), Biochim. Biophys. Acta, 1844, 1486-1492.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures in an apo-form at 2.00 A and NADPH-complexed form at 2.40 A resolution. Gre2 forms a homodimer, each subunit of which contains an N-terminal Rossmann-fold domain and a variable C-terminal domain, which participates in substrate recognition. The induced fit upon binding to the cofactor NADPH makes the two domains shift toward each other, producing an interdomain cleft that better fits the substrate Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
F132A about 200% of wild-type activity Saccharomyces cerevisiae
F85A less than 0.5% of wild-type activity Saccharomyces cerevisiae
L169L complete loss of activity Saccharomyces cerevisiae
S127A complete loss of activity Saccharomyces cerevisiae
V162A about 150% of wild-type activity Saccharomyces cerevisiae
V198A about 1.5% of wild-type activity Saccharomyces cerevisiae
Y128A about 1.5% of wild-type activity Saccharomyces cerevisiae
Y128F activity similar to wild-type Saccharomyces cerevisiae
Y165A complete loss of activity Saccharomyces cerevisiae
Y165F complete loss of activity Saccharomyces cerevisiae
Y198F about 75% of wild-type activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
3-Methylbutanal mutant Y198F, pH 7.5, 30°C Saccharomyces cerevisiae
0.14
-
3-Methylbutanal wild-type, pH 7.5, 30°C Saccharomyces cerevisiae
0.18
-
3-Methylbutanal mutant F132A, pH 7.5, 30°C Saccharomyces cerevisiae
0.2
-
3-Methylbutanal mutant V162A, pH 7.5, 30°C Saccharomyces cerevisiae
0.23
-
3-Methylbutanal mutant Y128F, pH 7.5, 30°C Saccharomyces cerevisiae
0.67
-
3-Methylbutanal mutant Y128A, pH 7.5, 30°C Saccharomyces cerevisiae
4.1
-
3-Methylbutanal mutant Y198A, pH 7.5, 30°C Saccharomyces cerevisiae
11.1
-
3-Methylbutanal mutant F85A, pH 7.5, 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12068 cf. EC 1.1.1.283
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylbutanal + NAD(P)H + H+
-
Saccharomyces cerevisiae 3-methylbutanol + NAD(P)+
-
r

Synonyms

Synonyms Comment Organism
Gre2
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
3-Methylbutanal mutant Y128A, pH 7.5, 30°C Saccharomyces cerevisiae
5.9
-
3-Methylbutanal mutant F85A, pH 7.5, 30°C Saccharomyces cerevisiae
9.3
-
3-Methylbutanal mutant Y198A, pH 7.5, 30°C Saccharomyces cerevisiae
15.5
-
3-Methylbutanal mutant Y198F, pH 7.5, 30°C Saccharomyces cerevisiae
23.2
-
3-Methylbutanal wild-type, pH 7.5, 30°C Saccharomyces cerevisiae
38.9
-
3-Methylbutanal mutant Y128F, pH 7.5, 30°C Saccharomyces cerevisiae
52.6
-
3-Methylbutanal mutant V162A, pH 7.5, 30°C Saccharomyces cerevisiae
62.4
-
3-Methylbutanal mutant F132A, pH 7.5, 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.53
-
3-Methylbutanal mutant F85A, pH 7.5, 30°C Saccharomyces cerevisiae
2.1
-
3-Methylbutanal mutant Y128A, pH 7.5, 30°C Saccharomyces cerevisiae
2.3
-
3-Methylbutanal mutant Y198A, pH 7.5, 30°C Saccharomyces cerevisiae
126.3
-
3-Methylbutanal mutant Y198F, pH 7.5, 30°C Saccharomyces cerevisiae
161
-
3-Methylbutanal wild-type, pH 7.5, 30°C Saccharomyces cerevisiae
166.6
-
3-Methylbutanal mutant Y128F, pH 7.5, 30°C Saccharomyces cerevisiae
261.5
-
3-Methylbutanal mutant V162A, pH 7.5, 30°C Saccharomyces cerevisiae
342.7
-
3-Methylbutanal mutant F132A, pH 7.5, 30°C Saccharomyces cerevisiae