Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
crystal structures in an apo-form at 2.00 A and NADPH-complexed form at 2.40 A resolution. Gre2 forms a homodimer, each subunit of which contains an N-terminal Rossmann-fold domain and a variable C-terminal domain, which participates in substrate recognition. The induced fit upon binding to the cofactor NADPH makes the two domains shift toward each other, producing an interdomain cleft that better fits the substrate | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
F132A | about 200% of wild-type activity | Saccharomyces cerevisiae |
F85A | less than 0.5% of wild-type activity | Saccharomyces cerevisiae |
L169L | complete loss of activity | Saccharomyces cerevisiae |
S127A | complete loss of activity | Saccharomyces cerevisiae |
V162A | about 150% of wild-type activity | Saccharomyces cerevisiae |
V198A | about 1.5% of wild-type activity | Saccharomyces cerevisiae |
Y128A | about 1.5% of wild-type activity | Saccharomyces cerevisiae |
Y128F | activity similar to wild-type | Saccharomyces cerevisiae |
Y165A | complete loss of activity | Saccharomyces cerevisiae |
Y165F | complete loss of activity | Saccharomyces cerevisiae |
Y198F | about 75% of wild-type activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
3-Methylbutanal | mutant Y198F, pH 7.5, 30°C | Saccharomyces cerevisiae | |
0.14 | - |
3-Methylbutanal | wild-type, pH 7.5, 30°C | Saccharomyces cerevisiae | |
0.18 | - |
3-Methylbutanal | mutant F132A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
0.2 | - |
3-Methylbutanal | mutant V162A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
0.23 | - |
3-Methylbutanal | mutant Y128F, pH 7.5, 30°C | Saccharomyces cerevisiae | |
0.67 | - |
3-Methylbutanal | mutant Y128A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
4.1 | - |
3-Methylbutanal | mutant Y198A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
11.1 | - |
3-Methylbutanal | mutant F85A, pH 7.5, 30°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12068 | cf. EC 1.1.1.283 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylbutanal + NAD(P)H + H+ | - |
Saccharomyces cerevisiae | 3-methylbutanol + NAD(P)+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Gre2 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
3-Methylbutanal | mutant Y128A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
5.9 | - |
3-Methylbutanal | mutant F85A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
9.3 | - |
3-Methylbutanal | mutant Y198A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
15.5 | - |
3-Methylbutanal | mutant Y198F, pH 7.5, 30°C | Saccharomyces cerevisiae | |
23.2 | - |
3-Methylbutanal | wild-type, pH 7.5, 30°C | Saccharomyces cerevisiae | |
38.9 | - |
3-Methylbutanal | mutant Y128F, pH 7.5, 30°C | Saccharomyces cerevisiae | |
52.6 | - |
3-Methylbutanal | mutant V162A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
62.4 | - |
3-Methylbutanal | mutant F132A, pH 7.5, 30°C | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
3-Methylbutanal | mutant F85A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
2.1 | - |
3-Methylbutanal | mutant Y128A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
2.3 | - |
3-Methylbutanal | mutant Y198A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
126.3 | - |
3-Methylbutanal | mutant Y198F, pH 7.5, 30°C | Saccharomyces cerevisiae | |
161 | - |
3-Methylbutanal | wild-type, pH 7.5, 30°C | Saccharomyces cerevisiae | |
166.6 | - |
3-Methylbutanal | mutant Y128F, pH 7.5, 30°C | Saccharomyces cerevisiae | |
261.5 | - |
3-Methylbutanal | mutant V162A, pH 7.5, 30°C | Saccharomyces cerevisiae | |
342.7 | - |
3-Methylbutanal | mutant F132A, pH 7.5, 30°C | Saccharomyces cerevisiae |