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Literature summary for 1.1.1.264 extracted from

  • Kuivanen, J.; Arvas, M.; Richard, P.
    Clustered genes encoding 2-keto-L-gulonate reductase and L-idonate 5-dehydrogenase in the novel fungal D-glucuronic acid pathway (2017), Front. Microbiol., 8, 225 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.264

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli TOP10 cells Aspergillus niger
expression in Saccharomyces cerevisiae Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.4
-
 5-Dehydro-D-gluconate pH 8, temperature not specified in the publication Aspergillus niger
8.4
-
 5-Dehydro-D-gluconate pH 7, temperature not specified in the publication Aspergillus niger
30.9
-
 L-Idonate pH 8, temperature not specified in the publication Aspergillus niger
30.9
-
 L-Idonate pH 7, temperature not specified in the publication Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-idonate + NAD+ Aspergillus niger the enzyme is part of the D-glucuronic acid catabolism 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+ Aspergillus niger ATCC 1015 the enzyme is part of the D-glucuronic acid catabolism 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+ Aspergillus niger CBS 113.46 the enzyme is part of the D-glucuronic acid catabolism 5-dehydro-D-gluconate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger ATCC 1015
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-dehydro-D-gluconate + NADH + H+
-
 Aspergillus niger L-idonate + NAD+
-
 ?
5-dehydro-D-gluconate + NADH + H+
-
 Aspergillus niger ATCC 1015 L-idonate + NAD+
-
 ?
5-dehydro-D-gluconate + NADH + H+
-
 Aspergillus niger CBS 113.46 L-idonate + NAD+
-
 ?
L-idonate + NAD+
-
 Aspergillus niger 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+ the enzyme is part of the D-glucuronic acid catabolism Aspergillus niger 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+ the enzyme has a strict requirement for NAD+/NADH Aspergillus niger 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+
-
 Aspergillus niger ATCC 1015 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+ the enzyme is part of the D-glucuronic acid catabolism Aspergillus niger ATCC 1015 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+ the enzyme has a strict requirement for NAD+/NADH Aspergillus niger ATCC 1015 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+
-
 Aspergillus niger CBS 113.46 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+ the enzyme is part of the D-glucuronic acid catabolism Aspergillus niger CBS 113.46 5-dehydro-D-gluconate + NADH + H+
-
 ?
L-idonate + NAD+ the enzyme has a strict requirement for NAD+/NADH Aspergillus niger CBS 113.46 5-dehydro-D-gluconate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GluE
-
 Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
 L-Idonate pH 8, temperature not specified in the publication Aspergillus niger
5.5
-
 L-Idonate pH 7, temperature not specified in the publication Aspergillus niger
7.2
-
 5-Dehydro-D-gluconate pH 8, temperature not specified in the publication Aspergillus niger
7.2
-
 5-Dehydro-D-gluconate pH 7, temperature not specified in the publication Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NAD+ strict requirement for NAD+/NADH as cofactors Aspergillus niger
NAD+ the enzyme has a strict requirement for NAD+/NADH Aspergillus niger
NADH strict requirement for NAD+/NADH as cofactors Aspergillus niger
NADH the enzyme has a strict requirement for NAD+/NADH Aspergillus niger

General Information

General Information Comment Organism
metabolism the enzyme is part of the D-glucuronic acid catabolism Aspergillus niger
physiological function GluE deletion results in reduced growth and cessation of the D-glucuronic acid catabolism Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.178
-
 L-Idonate pH 8, temperature not specified in the publication Aspergillus niger
0.857
-
 5-Dehydro-D-gluconate pH 7, temperature not specified in the publication Aspergillus niger