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Literature summary for 1.1.1.261 extracted from

  • Koga, Y.; Ohga, M.; Tsujimura, M.; Morii, H.; Kawarabayasi, Y.
    Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information on a hyperthermophilic archaeon, Sulfolobus tokodaii strain 7 (2006), Biosci. Biotechnol. Biochem., 70, 282-285.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
NADPH at 65°C, pH 6.5, in 50 mM MOPS buffer Sulfurisphaera tokodaii
0.47
-
dihydroxyacetone phosphate at 65°C, pH 6.5, in 50 mM MOPS buffer Sulfurisphaera tokodaii

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
SDS-PAGE Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate + NAD(P)H
-
Sulfurisphaera tokodaii sn-glycerol-1-phosphate + NAD(P)+
-
r

Synonyms

Synonyms Comment Organism
G-1-P-dehydrogenase
-
Sulfurisphaera tokodaii
sn-glycerol-1-phosphate dehydrogenase
-
Sulfurisphaera tokodaii
ST0344 protein
-
Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 85 at temperatures higher than 85°C no activity of the enzyme can be detected due to precipitation Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
in MES buffer or MOPS buffer Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
5.6 7.7
-
Sulfurisphaera tokodaii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.8
-
at pH 6.8 86-91% of activity is detected Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
Sulfurisphaera tokodaii