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Literature summary for 1.1.1.26 extracted from

  • Ching, S.L.; Gidda, S.K.; Rochon, A.; van Cauwenberghe, O.R.; Shelp, B.J.; Mullen, R.T.
    Glyoxylate reductase isoform 1 is localized in the cytosol and not peroxisomes in plant cells (2012), J. Integr. Plant Biol., 54, 152-168.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol GLYR1 is exclusively located in the cytosol Arabidopsis thaliana 5829
-
additional information GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycolate + NAD+ Arabidopsis thaliana
-
glyoxylate + NADH + H+
-
r
glycolate + NADP+ Arabidopsis thaliana
-
glyoxylate + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LSV0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolate + NAD+
-
Arabidopsis thaliana glyoxylate + NADH + H+
-
r
glycolate + NADP+
-
Arabidopsis thaliana glyoxylate + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
glyoxylate reductase isoform 1
-
Arabidopsis thaliana
GLYR1
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana
NADP+
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana

General Information

General Information Comment Organism
additional information due to the glutamate at the -1 position, GLYR1 C-terminal tripeptide, -SRE, does not function as a type 1 peroxisomal targeting signal, PTS1. GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress Arabidopsis thaliana
physiological function GLYR1 scavenges succinic semialdehyde and glyoxylate that escape from mitochondria and peroxisomes, respectively Arabidopsis thaliana