Cloned (Comment) | Organism |
---|---|
expressed as a glutathione S-transferase (GST)-fusion protein in Escherichia coli BL21 cells | Curvularia lunata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,7-dihydroxyflavone | - |
Curvularia lunata | |
3-hydroxyflavone | - |
Curvularia lunata | |
5,7-dihydroxyflavone | potent inhibitor of 3HNR | Curvularia lunata | |
5-hydroxyflavone | - |
Curvularia lunata | |
5-methoxyflavone | - |
Curvularia lunata | |
5-methyl-(1,2,4)-triazolo[3,4-b]benzothiazole | tricyclazole, specific inhibitor of 3HNR | Curvularia lunata | |
apigenin | potent inhibitor of 3HNR | Curvularia lunata | |
Biochanin A | - |
Curvularia lunata | |
kaempferol | potent inhibitor of 3HNR | Curvularia lunata | |
luteolin | potent inhibitor of 3HNR | Curvularia lunata | |
naringenin | - |
Curvularia lunata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3,8-trihydroxynaphthalene + NADPH + H+ | Curvularia lunata | - |
vermelone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curvularia lunata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by affinity binding followed by cleavage with thrombin | Curvularia lunata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3,8-trihydroxynaphthalene + NADPH + H+ | - |
Curvularia lunata | vermelone + NADP+ | - |
? | |
1,4-androstadiene-3,17-dione + NADPH + H+ | poor substrate of 3HNR | Curvularia lunata | ? + NADP+ | - |
? | |
2,(5alpha)-androstene-17-one + NADPH + H+ | - |
Curvularia lunata | ? + NADP+ | - |
? | |
2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one + NADP+ | - |
Curvularia lunata | 4,5-dihydroxy-2H-benzopyran-2-one + NADPH + H+ | - |
? | |
4-androstene-3,17-dione + NADPH + H+ | poor substrate of 3HNR | Curvularia lunata | ? + NADP+ | - |
? | |
4-estrene-3,17-dione + NADPH + H+ | poor substrate of 3HNR | Curvularia lunata | ? + NADP+ | - |
? | |
5-androstene-3beta-ol-17-one + NADPH + H+ | - |
Curvularia lunata | ? + NADP+ | - |
? | |
5alpha-androstane-3,17-dione + NADPH + H+ | best steroid substrate for 3HNR | Curvularia lunata | ? + NADP+ | - |
? | |
5alpha-androstane-3-one + NADPH + H+ | - |
Curvularia lunata | ? + NADP+ | - |
? | |
5alpha-androstane-3alpha-ol-17-one + NADPH + H+ | - |
Curvularia lunata | ? + NADP+ | - |
? | |
5alpha-androstane-3beta-ol-17-one + NADPH + H+ | - |
Curvularia lunata | ? + NADP+ | - |
? | |
additional information | no activity with estrone | Curvularia lunata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3HNR | - |
Curvularia lunata |
trihydroxynaphthalene reductase | - |
Curvularia lunata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | optimal temperature for oxidation of 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | optimal pH for oxidation of 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0031 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | apigenin | |
0.0058 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | 5,7-dihydroxyflavone | |
0.0068 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | luteolin | |
0.0079 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | kaempferol | |
0.012 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | Biochanin A | |
0.025 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | 3-hydroxyflavone | |
0.025 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | 5-hydroxyflavone | |
0.025 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | 3,7-dihydroxyflavone | |
0.025 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | 5-methoxyflavone | |
0.026 | - |
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one | Curvularia lunata | naringenin |