Cloned (Comment) | Organism |
---|---|
gene tm0297, located in an operon with tm0298 encoding a mannitol dehydrogenase, phylogenetic analysis, expression of the Strep-tagged enzyme in Escherichia coli | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
NADH | with D-ribulose, pH 7.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
0.031 | - |
NADH | with D-ribulose, pH 7.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
0.059 | - |
NAD+ | with D-arabinitol, pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
0.069 | - |
NAD+ | with D-arabinitol, pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
2.6 | - |
D-arabinitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
7.1 | - |
D-arabinitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
7.2 | - |
D-ribulose | pH 7.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
13.9 | - |
D-ribulose | pH 7.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
20 | - |
D-xylitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
21.7 | - |
D-xylitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | twofold activation at 60 mM, contributes to the overall stability of the protein and ise not required for catalytic activity | Thermotoga maritima | |
Mg2+ | twofold activation at 55 mM, contributes to the overall stability of the protein and is not required for catalytic activity | Thermotoga maritima | |
additional information | no effect on the activity by CaCl2 or NaCl | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27600 | - |
6 * 27600, SDS-PAGE | Thermotoga maritima |
164000 | - |
native PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
gene tm0297 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Thermotoga maritima cannot use D-arabitol as a sole carbon source. Optimal growth temperature of Thermotoga maritima is 80°C | Thermotoga maritima | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinitol + NAD+ | - |
Thermotoga maritima | D-ribulose + NADH + H+ | - |
r | |
D-xylitol + NAD+ | - |
Thermotoga maritima | D-xylulose + NADH + H+ | - |
r | |
additional information | the enzyme exclusively catalyzed the NAD(H)-dependent oxidoreduction of D-arabitol, D-xylitol, D-ribulose, or D-xylulose | Thermotoga maritima | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 27600, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
arabitol 2-dehydrogenase | - |
Thermotoga maritima |
ArDH | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 85 | inactive below 50°C, optimal activity at 85°C | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the addition of the Strep-tag led to a destabilization of tmArDH resulting in the loss of thermostability | Thermotoga maritima |
85 | - |
rapid inactivation of the recombinant Strep-tagged enzyme, while the detagged enzyme retains over 90% activity after 90 min at 85°C | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
D-arabinitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
11.2 | - |
D-xylitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
16.6 | - |
D-xylitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
18.7 | - |
D-arabinitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
165.6 | - |
D-ribulose | pH 7.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
193.2 | - |
D-ribulose | pH 7.5, 85°C, recombinant detagged enzyme | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reduction reaction | Thermotoga maritima |
8.5 | - |
oxidation reaction | Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | 40% and 10% of maximal activity at pH 5.5 and pH 8.5, no activity below pH 4.5 and above pH 9.5 | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Thermotoga maritima | |
NADH | dependent on | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
D-xylitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
0.74 | - |
D-xylitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
1.5 | - |
D-arabinitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
7.2 | - |
D-arabinitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
12 | - |
D-ribulose | pH 7.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
27 | - |
D-ribulose | pH 7.5, 85°C, recombinant detagged enzyme | Thermotoga maritima |