Cloned (Comment) | Organism |
---|---|
gene ydiB, the ydiB gene is cloned into plasmid pTOPO aroB aroE, resulting in the pTOPO ydiB aroB aroE derivative, enzyme overexpression in Escherichia coli strain PB12, quantitative RT-PCR analysis, coexpression of plasmid pTOPO aroB aroE and pJLB aroG fbr tktA and the cultivation of this derivative in Escherichia coli strain PB12.SA2 resulting in very high level expression of gene ydiB during exponential and stationary growth stages | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ydiB-encoded enzyme knockout in Escherichia coli strain PB12 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | Escherichia coli | - |
3-dehydroquinate + NADH + H+ | - |
r | |
L-quinate + NAD+ | Escherichia coli PB12 | - |
3-dehydroquinate + NADH + H+ | - |
r | |
shikimate + NAD+ | Escherichia coli | - |
3-dehydroshikimate + NADH + H+ | - |
r | |
shikimate + NAD+ | Escherichia coli PB12 | - |
3-dehydroshikimate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6D5 | - |
- |
Escherichia coli PB12 | P0A6D5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Escherichia coli strain PB12.SA22 and the derivatives ydiB- and ydiB+ are evaluated for their ability to produce shikimate (SA), quinate (QA), 3-dehydroshikimate (DHS), and 3-dehydroquinate (DHQ) in batch culture fermentations growing in 1-l fermentors using 500 ml of a mineral broth supplemented with 25 g/l glucose and 15 g/l YE. Biomass and glucose consumption and the production of aromatic intermediates of the SA pathway, SA, QA, DHQ, and DHS are determined for all derivatives, overview. The highest production of DHQ and DHS is 0.07 and 0.074 g/l, respectively. SA and QA are produced during the early exponential stage, as these compounds are detected during the first 5 h of cultivation (SA = 0.49 g/l and QA = 0.38 g/l, respectively). In the stationary stage and until 20 h of cultivation, this strain consumes the remaining residual glucose. From this time until the end of fermentation, the supernatant concentration of detected SA shows no significant changes, reaching 8.2 g/l by the end of fermentation (50 h), whereas the final QA concentration is 1.52 g/l | Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | - |
Escherichia coli | 3-dehydroquinate + NADH + H+ | - |
r | |
L-quinate + NAD+ | - |
Escherichia coli PB12 | 3-dehydroquinate + NADH + H+ | - |
r | |
shikimate + NAD+ | - |
Escherichia coli | 3-dehydroshikimate + NADH + H+ | - |
r | |
shikimate + NAD+ | - |
Escherichia coli PB12 | 3-dehydroshikimate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent enzyme quinate/shikimate dehydrogenase | - |
Escherichia coli |
quinate/shikimate dehydrogenase | - |
Escherichia coli |
YdiB | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | Escherichia coli constitutively expresses two shikimate dehydrogenase paralogues, AroE and the NAD+ -dependent enzyme quinate/shikimate dehydrogenase (YdiB), sharing 25% sequence identity. While AroE is NADP+-dependent, YdiB uses NADP+ or NAD+. Contrary to AroE, YdiB displays a clear activity on quinate, with either NADP+ or NAD+ as a cofactor in addition to shikimate | Escherichia coli |
malfunction | in the ydiB knockout mutant, QA production is 6.17% relative to SA (mol/mol), indicating that the inactivation of ydiB is a suitable strategy to reduce QA production below 10% (mol/mol) relative to SA in culture fermentations for SA production. The inactivation of ydiB in Escherichia coli strain PB12.SA22 and the reduction in QA production support the role of YdiB in the synthesis of this compound from DHQ. In the absence of YdiB, the DHS concentration detected in supernatant cultures is maintained relatively constant during the stationary phase | Escherichia coli |