Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli SG13009 | Papaver somniferum |
Protein Variants | Comment | Organism |
---|---|---|
D107A | increase in Km, 2fold decrease in kcat | Papaver somniferum |
F104A | increase in kcat | Papaver somniferum |
F104A/I275A | shows no substrate inhibition accompanied by a weak affinity for productive salutaridine binding, kcat is almost 2fold higher compared with the wild-type | Papaver somniferum |
I275A | increase in Km, shows an increase of velocity by a factor of 2.3 | Papaver somniferum |
K186V | moderate effects on substrate affinity, exhibits a turnover number similar to that of the wild-type | Papaver somniferum |
L185A | increase in Km, decrease in kcat | Papaver somniferum |
L266A | decrease in kcat | Papaver somniferum |
M271A | decrease in kcat | Papaver somniferum |
M271A | increase in Km | Papaver somniferum |
N272A | increase in Km, decrease in kcat | Papaver somniferum |
S181A | moderate effects on substrate affinity, increase in kcat by 50% | Papaver somniferum |
T182A | moderate effects on substrate affinity | Papaver somniferum |
V106A | moderate effects on substrate affinity, increase in kcat by 90% | Papaver somniferum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | strong substrate inhibition of SalR | Papaver somniferum | |
NADP+ | product inhibition | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
salutaridine | wild-type | Papaver somniferum | |
0.0034 | - |
NADPH | mutant K186V | Papaver somniferum | |
0.0035 | - |
NADPH | wild-type | Papaver somniferum | |
0.0041 | - |
salutaridine | mutant T182A | Papaver somniferum | |
0.0045 | - |
NADPH | mutant S181A | Papaver somniferum | |
0.005 | - |
salutaridine | mutant S181A | Papaver somniferum | |
0.007 | - |
NADPH | mutant V106A | Papaver somniferum | |
0.0078 | - |
salutaridine | mutant K186V | Papaver somniferum | |
0.0089 | - |
salutaridine | mutant V106A | Papaver somniferum | |
0.0097 | - |
NADPH | mutant F104A | Papaver somniferum | |
0.0132 | - |
salutaridine | mutant L185A | Papaver somniferum | |
0.0148 | - |
salutaridine | mutant D107A | Papaver somniferum | |
0.0155 | - |
NADPH | mutant L266A | Papaver somniferum | |
0.0156 | - |
NADPH | mutant N272A | Papaver somniferum | |
0.0198 | - |
NADPH | mutant M271A | Papaver somniferum | |
0.0211 | - |
NADPH | mutant I275A | Papaver somniferum | |
0.0213 | - |
NADPH | mutant T182A | Papaver somniferum | |
0.0225 | - |
salutaridine | mutant M271A | Papaver somniferum | |
0.0297 | - |
NADPH | mutant L185A | Papaver somniferum | |
0.0305 | - |
NADPH | mutant D107A | Papaver somniferum | |
0.0318 | - |
salutaridine | mutant F104A | Papaver somniferum | |
0.0331 | - |
salutaridine | mutant I275A | Papaver somniferum | |
0.043 | - |
NADPH | mutant F104A/I275A | Papaver somniferum | |
0.0494 | - |
salutaridine | mutant L266A | Papaver somniferum | |
0.0901 | - |
salutaridine | mutant N272A | Papaver somniferum | |
0.4086 | - |
salutaridine | mutant F104A/I275A | Papaver somniferum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | Q071N0 | - |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Papaver somniferum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
salutaridine + NADPH + H+ | - |
Papaver somniferum | (7S)-salutaridinol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SalR | - |
Papaver somniferum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
salutaridine | mutant M271A | Papaver somniferum | |
0.016 | - |
salutaridine | mutant L185A | Papaver somniferum | |
0.021 | - |
salutaridine | mutant N272A | Papaver somniferum | |
0.126 | - |
salutaridine | mutant T182A | Papaver somniferum | |
0.501 | - |
salutaridine | mutant L266A | Papaver somniferum | |
0.61 | - |
salutaridine | mutant D107A | Papaver somniferum | |
0.998 | - |
salutaridine | mutant K186V | Papaver somniferum | |
1.306 | - |
salutaridine | wild-type | Papaver somniferum | |
1.933 | - |
salutaridine | mutant S181A | Papaver somniferum | |
2.45 | - |
salutaridine | mutant F104A/I275A | Papaver somniferum | |
2.458 | - |
salutaridine | mutant V106A | Papaver somniferum | |
2.727 | - |
salutaridine | mutant F104A | Papaver somniferum | |
3.188 | - |
salutaridine | mutant I275A | Papaver somniferum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Papaver somniferum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
NADP+ | - |
Papaver somniferum |