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Literature summary for 1.1.1.244 extracted from

  • Yi, J.; Lee, J.; Sung, B.H.; Kang, D.K.; Lim, G.; Bae, J.H.; Lee, S.G.; Kim, S.C.; Sohn, J.H.
    Development of Bacillus methanolicus methanol dehydrogenase with improved formaldehyde reduction activity (2018), Sci. Rep., 8, 12483 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Bacillus methanolicus

Protein Variants

Protein Variants Comment Organism
F213V/F289L the mutant enzyme shows 25.3fold higher catalytic efficiency (kcat/Km) than wild type enzyme. It converts 5.9fold more formaldehyde to methanol in vitro than the wild type enzyme Bacillus methanolicus
F213V/F289L/F356S the mutant enzyme shows 52.8fold higher catalytic efficiency (kcat/Km) than wild type enzyme. It converts 6.4fold more formaldehyde to methanol in vitro than the wild type enzyme Bacillus methanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + NADH + H+ Bacillus methanolicus key step for both toxic formaldehyde elimination methanol + NAD+
-
r
formaldehyde + NADH + H+ Bacillus methanolicus C1 key step for both toxic formaldehyde elimination methanol + NAD+
-
r
methanol + NAD+ Bacillus methanolicus key step for ethanol production in bacterial methylotrophy formaldehyde + NADH + H+
-
r
methanol + NAD+ Bacillus methanolicus C1 key step for ethanol production in bacterial methylotrophy formaldehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus P31005
-
-
Bacillus methanolicus C1 P31005
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus methanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + NADH + H+
-
Bacillus methanolicus methanol + NAD+
-
r
formaldehyde + NADH + H+ key step for both toxic formaldehyde elimination Bacillus methanolicus methanol + NAD+
-
r
formaldehyde + NADH + H+
-
Bacillus methanolicus C1 methanol + NAD+
-
r
formaldehyde + NADH + H+ key step for both toxic formaldehyde elimination Bacillus methanolicus C1 methanol + NAD+
-
r
methanol + NAD+
-
Bacillus methanolicus formaldehyde + NADH + H+
-
r
methanol + NAD+ key step for ethanol production in bacterial methylotrophy Bacillus methanolicus formaldehyde + NADH + H+
-
r
methanol + NAD+
-
Bacillus methanolicus C1 formaldehyde + NADH + H+
-
r
methanol + NAD+ key step for ethanol production in bacterial methylotrophy Bacillus methanolicus C1 formaldehyde + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
MDH
-
Bacillus methanolicus

General Information

General Information Comment Organism
evolution the formaldehyde reduction activity of the enzyme is successfully improved by directed evolution and screening, which might potentially be useful for the conversion of CO2 to methanol Bacillus methanolicus
metabolism the enzyme catalyzes a key step for ethanol production in bacterial methylotrophy and a key step for both toxic formaldehyde elimination Bacillus methanolicus