Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Bacillus methanolicus |
Protein Variants | Comment | Organism |
---|---|---|
F213V/F289L | the mutant enzyme shows 25.3fold higher catalytic efficiency (kcat/Km) than wild type enzyme. It converts 5.9fold more formaldehyde to methanol in vitro than the wild type enzyme | Bacillus methanolicus |
F213V/F289L/F356S | the mutant enzyme shows 52.8fold higher catalytic efficiency (kcat/Km) than wild type enzyme. It converts 6.4fold more formaldehyde to methanol in vitro than the wild type enzyme | Bacillus methanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NADH + H+ | Bacillus methanolicus | key step for both toxic formaldehyde elimination | methanol + NAD+ | - |
r | |
formaldehyde + NADH + H+ | Bacillus methanolicus C1 | key step for both toxic formaldehyde elimination | methanol + NAD+ | - |
r | |
methanol + NAD+ | Bacillus methanolicus | key step for ethanol production in bacterial methylotrophy | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Bacillus methanolicus C1 | key step for ethanol production in bacterial methylotrophy | formaldehyde + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | P31005 | - |
- |
Bacillus methanolicus C1 | P31005 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus methanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NADH + H+ | - |
Bacillus methanolicus | methanol + NAD+ | - |
r | |
formaldehyde + NADH + H+ | key step for both toxic formaldehyde elimination | Bacillus methanolicus | methanol + NAD+ | - |
r | |
formaldehyde + NADH + H+ | - |
Bacillus methanolicus C1 | methanol + NAD+ | - |
r | |
formaldehyde + NADH + H+ | key step for both toxic formaldehyde elimination | Bacillus methanolicus C1 | methanol + NAD+ | - |
r | |
methanol + NAD+ | - |
Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | key step for ethanol production in bacterial methylotrophy | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Bacillus methanolicus C1 | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | key step for ethanol production in bacterial methylotrophy | Bacillus methanolicus C1 | formaldehyde + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Bacillus methanolicus |
General Information | Comment | Organism |
---|---|---|
evolution | the formaldehyde reduction activity of the enzyme is successfully improved by directed evolution and screening, which might potentially be useful for the conversion of CO2 to methanol | Bacillus methanolicus |
metabolism | the enzyme catalyzes a key step for ethanol production in bacterial methylotrophy and a key step for both toxic formaldehyde elimination | Bacillus methanolicus |