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Literature summary for 1.1.1.244 extracted from

  • Whitaker, W.; Jones, J.; Bennett, R.; Gonzalez, J.; Vernacchio, V.; Collins, S.; Palmer, M.; Schmidt, S.; Antoniewicz, M.; Koffas, M.; Papoutsakis, E.
    Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli (2017), Metab. Eng., 39, 49-59 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli strain BW25113 DELTAfrmA by expressing the methanol dehydrogenase from Bacillus stearothermophilus and and ribulose monophosphate (RuMP) pathway enzymes from Bacillus methanolicus. The recombinant Escherichia coli strain converts methanol into biomass components. Effective methanol assimilation by the engineered Escherichia coli strain can be enhanced in the presence of small amounts of yeast extract or tryptone. Method, overview Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + NAD+ Geobacillus stearothermophilus
-
formaldehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + NAD+
-
Geobacillus stearothermophilus formaldehyde + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
BsMdh
-
Geobacillus stearothermophilus
MDH
-
Geobacillus stearothermophilus
NAD-dependent MDH
-
Geobacillus stearothermophilus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
additional information
-
thermodynamics for NAD-dependent methanol oxidation are unfavorable at low temperatures but become more favorable at higher temperatures Geobacillus stearothermophilus
37
-
assay at Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Geobacillus stearothermophilus

General Information

General Information Comment Organism
metabolism assimilation of methanol into central metabolism, overview Geobacillus stearothermophilus